Effect of Fermentation Broth from Indole-3-Acetic Acid (IAA) Producing Methylobacterium radiotolerans ED5-9 on the Growth and Development of Murdannia loriformis (Hassk.) Rolla Rao & Kammathy under In vitro Condition
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Abstract
The possible role of indole-3-acetic acid (IAA) produced from Methylobacterium radiotolerans ED5-9 has been studied in the tissue culture of Murdannia loriformis for 4 weeks. The concentration of IAA in liquid nutrient medium (LNM) of M. radiotolerans ED5-9 culture was 3.36 ± 0.20 µg/ml. This supernatant was mixed with Murashige and skoog (MS) medium in varying volumes (1, 2 and 4 ml/l) and compared with MS medium supplemented with IAA for the tissue culture of M. loriformis. Results show that bacterial IAA can promote plant growth in terms of increasing number of shoots, roots and leaves as well increases in root length and dry weight more than chemically synthesized IAA. Root formation and increase in root length was found to be highest in treatments having 2 ml/l bacterial supernatant in 0.8% agar medium with an average of 11.00 ± 4.60 roots per explants and 2.10 ± 0.70 cm, respectively. In addition, Total phenolic compounds and antioxidant activity were found in quantity as 0.36 ± 0.46 and 4.14 ± 0.52 mg/g fresh weight, respectively. As the concentration of IAA in supernatant from M. radiotolerans ED5-9 was increased, the number of roots also increased.
Keywords: indole-3-acetic acid (IAA), Methylobacterium radiotolerans ED5-9, Murdannia loriformis, Tissue culture
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