Effect of Methylobacterium radiotolerans ED5-9 with Capability of Producing Indole-3-Acetic Acid (IAA) and 1-Aminocyclopropane-1-Carboxylic Acid Deaminase on the Growth and Development of Murdannia loriformis (Hassk.) Rolla Rao & Kammathy under In Vitro Condition
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Published
Apr 26, 2017
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Abstract
This study aimed to evaluate the production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzyme from endophytic Methylobacterium radiotolerans ED5-9, and to investigate the effects of M. radiotolerans ED5-9 on Murdannia loriformis, under tissue culture condition. M. radiotolerans ED5-9 was isolated from leaves of M. loriformis and cultured in vitro. It produced indole-3-acetic acid (IAA) with a concentration of 3.36 ± 0.20 µg/ml after incubation for 60 hr. Activity of ACC deaminase enzyme was observed at 365.05 ± 90.51 nmol of a-ketobutyrate/mg of protein/h. To test the effects of inoculation, the experiment was carried on by immersion of the explants of M. loriformis into M. radiotolerans ED5-9 suspension for 1, 3 and 5 min, and subsequently cultured on the MS medium in which 2 mg/l of IAA substance was used as the control. The results showed that duration time of the immersed explants in M. radiotolerans ED5-9 suspension for 5 min had the highest average number of shoots (6.00 ± 1.00 shoots per explants), root length (0.71 ± 0.26 cm) and dry weight (46.00 ± 5.40 mg). However, the above results of growth were similar to the growth of M. loriformis explants in the control. The results indicated the possible utilizing of M. radiotolerans ED5-9 can produce ACC deaminase enzyme and IAA to enhance growth and development of the M. loriformis explants under the in vitro condition.
References
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Cervantes, S. E., Graham, E. A., & Andrade, J. L. (2005). Light microhabitats, growth and photosynthesis of an epiphytic bromeliad in a tropical dry forest. Plant Ecology, 179(1), 107-118.
Chinnadurai, C., Balachandar, D., & Sundaram, S. P. (2009). Characterization of 1 aminocyclopropane-1-carboxylate deaminase producing methylobacteria from phyllosphere of rice and their role in ethylene regulation. Word Journal of Microbiology and Biotechnology, 25(8), 1403-1411.
Debnath, M., Malik, C. P., & Bisen, P. S. (2006). Micropropagation: a tool for the production of high quality plant-based medicines. Current Pharmaceutical Biotechnology, 7(1), 33-49.
Devi, R. P., Sundaram, S. P., & Poorniammal, R. (2010). Effect of facultative methyltrophs on tissue culturing of rice. Asian Journal of Bio Science, 4(2), 207-209.
Dileepkumar, B. S., & Dube, H. C. (1992). Seed bacterization with fluorescent Pseudomonas for enhanced plant growth, yield and disease control. Soil Biology and Biochemistry, 24(6), 539-542.
Doronina, N. V., Ivanova, E. G., & Trotsenko, Y. A. (2002). New evidence for the ability of methylobacteria and methanotrophs to synthesize auxins. Microbiology, 71(1), 130-132.
Glick, B. R., Penrose, D. M., & Li, J. (1998). Model for the lowering of plant ethylene concentration by plant growth-promoting bacteria. Journal of Theoretical Biology, 190(1), 63-68.
Glick, B. R., Todorovic, B., Czarny, J., Cheng, Z., Duan, J., & McConkey, B. (2007). Promotion of plant growth by bacterial ACC deaminase. Critical Review in Plant Sciences, 26(5-6), 227-242.
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Hsh, C. Y. (2006). Antioxidant activity of extract from Polygonum aviculare L. Biological Research, 39(2), 281-288.
Ivanova, E. G., Doronina, N. V., & Trotsenko, Y. A. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology, 70(4), 452-458.
Jiratchariyakul, W., Okabe, H., Moongkarndi, P., & Frahm, A. W. (1998). Cytotoxic Glycosphingolipid from Murdannia loriformis (Hassk.) Rolla Rao & Kam. Thai Journal of Phytopharmacy, 5(1), 10-20.
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Kalyaeva, M. A., Ivanova, E. G., Doronina, N. V., Zakharchenko, N. S., Trotsenko, Y. A., & Buryanov, Y. I. (2003). The Effect of aerobic methylotrophic bacteria on the In vitro morphogenesis of soft wheat (Triticum aestivum). Russian Journal of Plant Physiology, 50(3), 313-317.
Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In Wrolstad, R. E., Acree, T. E., Decker, E. A., Penner, M. H., Reid, D. S., Schwartz, S. J., Shoemaker, C. F., Smith, D. M., & Sporns, P. (Eds.), Current Protocols in Food Analytical Chemistry (pp. F4.3.1-F4.3.8). N.P.: John Wiley and Sons.
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Madhaiyan, M., Poonguzhali, S., Ryu, J., & Sa, T. (2006). Regulation of ethylene levels in Canola (Brassica campestris) by 1-Aminocyclopropane 1-carboxylate deaminase-containing Methylobacterium fujisawaense. Planta, 224(2), 268–278.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15(3), 473-497.
Neumann, K. H., Kumar, A., & Imani, J. (2009). Plant cell and tissue culture- A tool in biotechnology: basics and application. Berlin, Heidelberg, Germany: Springer-Verlag.
Penrose, D. M., & Glick, B. R. (2001). Levels of 1-aminocyclopropane-1-carboxylic acid (ACC) in exudates and extracts of canola seeds treated with plant growth-promoting bacteria. Canadian Journal of Microbiology, 47(4), 368-372.
Penrose, D. M., & Glick, B. R. (2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum, 118(1), 10-15.
Pongsathorn, K., Duangporn, P., Sireethon, K., & Pornchanok, C. (2012). Determination of antioxidant property from some medicinal plant extracts from Thailand. African Journal of Biotechnology, 11(45), 10322-10327.
Prinsen, E., Costacurta, A., Michiels, K., Vanderleyden, J., & Onckelen, H. V. (1993). Azosipirillum brasilense indole-3-acetic acid biosynthesis: evidence for a non-tryptophandependent pathway. Molecular Plant-Microbe Interacts, 6(5), 609-915.
Rao, S. R., & Ravishankar, G. A. (2002). Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20(2), 101-153.
Russo, A., Vettori, L., Felici, C., Fiaschi, G., Morini, S., & Toffanin, A. (2008). Enhanced micropropagation response and biocontrol effect of Azospirillum brasilense Sp245 on Prunus cerasifera L. Clone Mr.S2/5 plants. Journal of Biotechnology, 134(3-4), 312-319.
Sarin, S., Prombunchachai, T., Nakaew, N., & Chidburee, A. (2013) Isolation of indole acetic acid producing pink pigmented facultative methylotrophs (PPFMs) from Murdannia loriformis (Hassk.) R. Rao & Kammathy. Naresuan University Journal, 21(2), 14-24.
Shirokikh, I. G., Shupletsova, O. N., & Shirokikh, A. A. (2007) Assessment of the effect of methylotrophic bacteria on plants In vitro. Russian Agricultural Sciences, 33(5), 308–310.
Arroo, R. R. J., Develi, A., Meijers, H., Van de Westerlo, E., Kemp, A. K., Croes, A. F., & Wullems, G. J. (1995). Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures. Physiologia Plantarum, 93(2), 233-240.
Cervantes, S. E., Graham, E. A., & Andrade, J. L. (2005). Light microhabitats, growth and photosynthesis of an epiphytic bromeliad in a tropical dry forest. Plant Ecology, 179(1), 107-118.
Chinnadurai, C., Balachandar, D., & Sundaram, S. P. (2009). Characterization of 1 aminocyclopropane-1-carboxylate deaminase producing methylobacteria from phyllosphere of rice and their role in ethylene regulation. Word Journal of Microbiology and Biotechnology, 25(8), 1403-1411.
Debnath, M., Malik, C. P., & Bisen, P. S. (2006). Micropropagation: a tool for the production of high quality plant-based medicines. Current Pharmaceutical Biotechnology, 7(1), 33-49.
Devi, R. P., Sundaram, S. P., & Poorniammal, R. (2010). Effect of facultative methyltrophs on tissue culturing of rice. Asian Journal of Bio Science, 4(2), 207-209.
Dileepkumar, B. S., & Dube, H. C. (1992). Seed bacterization with fluorescent Pseudomonas for enhanced plant growth, yield and disease control. Soil Biology and Biochemistry, 24(6), 539-542.
Doronina, N. V., Ivanova, E. G., & Trotsenko, Y. A. (2002). New evidence for the ability of methylobacteria and methanotrophs to synthesize auxins. Microbiology, 71(1), 130-132.
Glick, B. R., Penrose, D. M., & Li, J. (1998). Model for the lowering of plant ethylene concentration by plant growth-promoting bacteria. Journal of Theoretical Biology, 190(1), 63-68.
Glick, B. R., Todorovic, B., Czarny, J., Cheng, Z., Duan, J., & McConkey, B. (2007). Promotion of plant growth by bacterial ACC deaminase. Critical Review in Plant Sciences, 26(5-6), 227-242.
Glickmann, E., & Dessaux, Y. (1995). A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61(2), 793-796.
Green, P. N. (2006). Methylobacterium. In M. Dworkin, S. Falkow, E. Rosenberg, K. Schleifer, & E. Stackebrandt (Eds.), The Prokaryotes Vol.5 (pp. 257-265). Singapore: Springer Science+Business Media LLC.
Holland, M. A. (1997). Occam’s razor applied to hormonology: are cytokinins produced by plants? Plant Physiology, 115(3), 865-868.
Holland, M. A., & Polacco, J. C. (1994). PPFMs and other covert contaminants: is there more to plant physiological than just plant? Annual Review of Plant Physiology and Plant Molecular Biology, 45(1), 197-209.
Hsh, C. Y. (2006). Antioxidant activity of extract from Polygonum aviculare L. Biological Research, 39(2), 281-288.
Ivanova, E. G., Doronina, N. V., & Trotsenko, Y. A. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology, 70(4), 452-458.
Jiratchariyakul, W., Okabe, H., Moongkarndi, P., & Frahm, A. W. (1998). Cytotoxic Glycosphingolipid from Murdannia loriformis (Hassk.) Rolla Rao & Kam. Thai Journal of Phytopharmacy, 5(1), 10-20.
Jiratchariyakul, W., Vongsakul, M., Sunthornsuk, L., Moongkarndi, P., Narintorn, A. Somanabandhu, A., Okabe, H., & Frahm, A. W. (2006). Immunomodulatory Effect and Quantitation of Cytotoxic Glycosphingolipid from Murdannia loriformis. Journal of Natural Medicines, 60(3), 210-216.
Kalyaeva, M. A., Ivanova, E. G., Doronina, N. V., Zakharchenko, N. S., Trotsenko, Y. A., & Buryanov, Y. I. (2003). The Effect of aerobic methylotrophic bacteria on the In vitro morphogenesis of soft wheat (Triticum aestivum). Russian Journal of Plant Physiology, 50(3), 313-317.
Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In Wrolstad, R. E., Acree, T. E., Decker, E. A., Penner, M. H., Reid, D. S., Schwartz, S. J., Shoemaker, C. F., Smith, D. M., & Sporns, P. (Eds.), Current Protocols in Food Analytical Chemistry (pp. F4.3.1-F4.3.8). N.P.: John Wiley and Sons.
Madhaiyan, M., Poonguzhali, S., & Sa, T. (2007). Characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing Methylobacterium oryzae and interactions with auxin and ACC regulation of ethylene in Canola (Brassica campestris). Planta, 226(4), 867-876.
Madhaiyan, M., Poonguzhali, S., Ryu, J., & Sa, T. (2006). Regulation of ethylene levels in Canola (Brassica campestris) by 1-Aminocyclopropane 1-carboxylate deaminase-containing Methylobacterium fujisawaense. Planta, 224(2), 268–278.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15(3), 473-497.
Neumann, K. H., Kumar, A., & Imani, J. (2009). Plant cell and tissue culture- A tool in biotechnology: basics and application. Berlin, Heidelberg, Germany: Springer-Verlag.
Penrose, D. M., & Glick, B. R. (2001). Levels of 1-aminocyclopropane-1-carboxylic acid (ACC) in exudates and extracts of canola seeds treated with plant growth-promoting bacteria. Canadian Journal of Microbiology, 47(4), 368-372.
Penrose, D. M., & Glick, B. R. (2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum, 118(1), 10-15.
Pongsathorn, K., Duangporn, P., Sireethon, K., & Pornchanok, C. (2012). Determination of antioxidant property from some medicinal plant extracts from Thailand. African Journal of Biotechnology, 11(45), 10322-10327.
Prinsen, E., Costacurta, A., Michiels, K., Vanderleyden, J., & Onckelen, H. V. (1993). Azosipirillum brasilense indole-3-acetic acid biosynthesis: evidence for a non-tryptophandependent pathway. Molecular Plant-Microbe Interacts, 6(5), 609-915.
Rao, S. R., & Ravishankar, G. A. (2002). Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20(2), 101-153.
Russo, A., Vettori, L., Felici, C., Fiaschi, G., Morini, S., & Toffanin, A. (2008). Enhanced micropropagation response and biocontrol effect of Azospirillum brasilense Sp245 on Prunus cerasifera L. Clone Mr.S2/5 plants. Journal of Biotechnology, 134(3-4), 312-319.
Sarin, S., Prombunchachai, T., Nakaew, N., & Chidburee, A. (2013) Isolation of indole acetic acid producing pink pigmented facultative methylotrophs (PPFMs) from Murdannia loriformis (Hassk.) R. Rao & Kammathy. Naresuan University Journal, 21(2), 14-24.
Shirokikh, I. G., Shupletsova, O. N., & Shirokikh, A. A. (2007) Assessment of the effect of methylotrophic bacteria on plants In vitro. Russian Agricultural Sciences, 33(5), 308–310.
Keywords
Methylobacterium radiotolerans, Murdannia loriformis, indole-3-acetic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, in vitro
Section
Research Articles
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How to Cite
PROMBUNCHACHAI, Thanawut et al.
Effect of Methylobacterium radiotolerans ED5-9 with Capability of Producing Indole-3-Acetic Acid (IAA) and 1-Aminocyclopropane-1-Carboxylic Acid Deaminase on the Growth and Development of Murdannia loriformis (Hassk.) Rolla Rao & Kammathy under In Vitro Condition.
Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 25, n. 2, p. 21-31, apr. 2017.
ISSN 2539-553X.
Available at: <https://www.journal.nu.ac.th/NUJST/article/view/1771>. Date accessed: 19 apr. 2024.