Isolation and Characterization of Bacteriophage which Infected Klebsiella pneumoniae

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Sunsiree Muangman Jiraporn Gatedee

Abstract

Klebsiella pneumoniae is a clinically significant organism that has caused much public health concern. Due to the increasing prevalence of infections caused by multidrug-resistant K. pneumoniae, alternative therapies might be promising. The properties of host specificity, abundance in nature, and evolvability suggest bacteriophages might be a good candidate. In this study, we have isolated and characterized a lytic bacteriophage infecting K. pneumoniae, ØKN-2, which was isolated from wastewater. The transmission electron micrograph showed that ØKN-2 was classified as being in the Myoviridae family. Host-range determination revealed that ØKN-2 is specifically able to lyse 35.7% (5/14) of tested K. pneumoniae. Studies of the host range of different species showed that ØKN-2 unable to lyse other tested bacteria including Acinetobacter baumannii, Peudomonas aeruginosa, Protues mirabilis, Enterococcus faecalis, and Escherichia coli. Interestingly, ØKN-2 significantly reduced K. pneumoniae ATCC 700603 biofilm preformed in a dose dependent manner. Therefore, this study identified and tested bacteriophages that infect  K. pneumoniae and proved the effectiveness of ØKN-2 against biofilm and host-specific characteristics which indicates that they are beneficial in the development of treatments against K. pneumoniae infections.


Keywords: K. pneumoniae, bacteriophages, biofilm, Myoviridae

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Research Articles

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How to Cite
MUANGMAN, Sunsiree; GATEDEE, Jiraporn. Isolation and Characterization of Bacteriophage which Infected Klebsiella pneumoniae. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 30, n. 4, p. 94-103, sep. 2022. ISSN 2539-553X. Available at: <https://www.journal.nu.ac.th/NUJST/article/view/Vol-30-No-4-2022-94-103>. Date accessed: 27 jan. 2023. doi: https://doi.org/10.14456/nujst.2022.39.