In Vitro Evaluation of Probiotic Potential of Novel Isolates of Lactobacillus from Native Pig Feces


Chiraprapha Tuyarum Benyapa Prakit Rungravee Chaiyod Thanchanok Suttibul Monthon Lertworapreecha


        The native pigs are a potential source of lactic acid bacteria (LAB) with probiotic properties since animal feed diversity plays a significant role in the intestinal tract's bacterial population. Usually, intestinal bacteria's activity promotes digestion, strengthens the immune system, and reduces deleterious microorganisms. These bacterial populations in the intestinal tract contain a probiotic group, of which the most qualified bacteria constitute LAB. This study proposes screening LAB and testing probiotics' properties from 25 feces of individual healthy native pigs. From the 139 isolates selected from feces samples, the antimicrobial activity inhibits pathogenic bacteria such as Enterohemorrhagic Escherichia coli (EHEC) isolated strain SC451-1, Enteropathogenic Escherichia coli (EPEC) isolated strain SC451-2, Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa ATCC27853, and Salmonella Typhimurium isolated strain SC2451-3, entirely tolerant at 1.0 % bile salt. Moreover, some of them could survive at a low pH of three and have a high hydrophobicity potential. This study, shown four isolates of the LAB high probiotic properties, and sequencing analysis indicated that four isolates were L. plantarum,
L salivarius
, L. paracasei, and L. paraplantarum. These properties allow the LAB to be considered a hopeful probiotic candidate for a feed additive of pig.

Keywords: Probiotics Properties, Lactobacillus spp, Pig feces


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Research Articles


How to Cite
TUYARUM, Chiraprapha et al. In Vitro Evaluation of Probiotic Potential of Novel Isolates of Lactobacillus from Native Pig Feces. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 30, n. 1, p. 42-52, may 2021. ISSN 2539-553X. Available at: <>. Date accessed: 18 jan. 2022.