Effects of Explants on Plant Regeneration and Concentration of Paclobutrazol on Morphological Responses of Dwarf Water Hyssop (Bacopa monnieri)

##plugins.themes.bootstrap3.article.main##

Chanraksmey Tauk Pornpimon Chuaduangpui Tassanee Khawniam

Abstract

Water hyssop is an ornamental aquatic plant which is very popular among aquarium hobbyists. The present study reports on the effect of PBZ concentration on water hyssop to produce dwarf aquatic plants. The different types of explant were cultured on MS medium supplemented with 0.5 mg/L BA for shoot and root induction. The single nodal was cut and cultured on MS supplemented with various PBZ concentrations to produce the dwarf ornamental aquatic plant. The results showed that shoot induction started within 2 weeks and the maximum average number of shoots received from the node with leaf explant was 12.11 shoots after 4 weeks of culturing. The highest concentration at 40 mg/L of PBZ retarded stems, number of leaves, number of roots, root length, and the survival rate declined within 2 weeks of culturing. Only 2.5 mg/ L PBZ stimulated shoot induction at 51. 85%, but 20 mg/ L of PBZ the percentage of shoot induction of 48.15 was obtained. Shoot length did not increase after treating with 40 mg/ L of PBZ and also resulted in the lowest average number of leaves of 1.25 leaves. The maximum concentration of PBZ caused toxic reactions, such as yellowing, stunted leaves, short roots, and death of in vitro cultures. The shoot length decreased with increasing concentrations of PBZ. Thus, at 2.5 mg/ L of PBZ was suitable for producing dwarf water hyssop plant with the maximum shoot length, maximum number of leave and longest root. Finally, the dwarf plantlets were acclimatized successfully to 4 weeks in aquarium conditions.

References

Boontiang, K., Chutichudet, B., & Chutichudet, P. (2019). Effect of paclobutrazol on growth and development of Curcuma alismatifolia Gagnep. Grown Off-season. Naresuan University Journal: Science and Technology, 27(1), 1-8. http://doi.org/10.14456/nujst.2019.1
Burrows, G. E., Boag, T. S., & Stewart, W. P. (1992). Changes in leaf, stem, and root anatomy of Chrysanthemum cv. Lillian Hoek following paclobutrazol application. Journal of Plant Growth Regulation, 11, 189–194. http://doi.org/10.1007/BF02115476
El-Fadl, R. E. S. A. (2017). Effect of growth retardants on shoot and root development of Stevia (Steviarebaudiana bertoni) plant grown in vitro. IOSR Journal of Agriculture and Veterinary Science 10, 16–24. http://dx.doi.org/10.9790/2380-1002011624
Evans, D. A., Sharp, W. R., Ammirato, P. V., & Yamada, Y. (1983). Handbook of plant cell culture, Technique for Propagation and Breeding. New York, London: Macmillan Publishing Company.
Indrayanti, R., Putri, R. E., Sedayu, A., & Adisyahputra. (2019). Effects of paclobutrazol for in vitro midium-term storage of banana variant cv. Kepok (Musa acuminata x balbisiana Colla). The 9th International Conference on Global Resource Conservation (ICGRC) and AJI from Ritsumeikan University. Retrieved from https://aip.scitation.org/doi/abs/10.1063/1.5061845
Joshi, A., Pathak, A. R., Sharma, A. M., & Singh, S. (2010). High frequency of shoot regeneration of leaf explants of Bacopa monnieri. Environmental and Experimental Biology, 8, 81-84. Retrieved from https://www.researchgate.net/
Karataș, M., & Aasim, M. (2014). Efficient in vitro regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell). Pakistan Journal of Agricultural Sciences, 51(3), 667-672. Retrieved from http://www.pakjas.com.pk/
Karatas, M., Aasim, M., Dogan, M., & Khawar, K. M. (2013). Adventitious shoot regeneration of the medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell) using different internodes. Archives of Biological Scieces Arhiv Za Bioloske Nauke, 65, 297-303. http://doi.org/10.2298/ABS130 1297K
Kuai, J., Li, X. Y., Yang, Y., & Zhou, G. S. (2017). Effects of paclobutrazol on biomass production in relation to resistance to lodging and pod shattering in Brassica napus L. Journal of Integrative Agriculture, 16, 2470–2481. http://doi.org/10.1016/S2095-3119(17)61674-5
Laohavisuti, N., Ruangdej, U., & Wangwibulkit, S. (2017). Effect of Kinetin and IAA on growth and antioxidants in Bacopa monnieri. King Mongkut’s Agricultural Journal, 35(2), 76-83. Retrieved from http://li01.tci-thaijo.org/index.php/agritechjournal/search/search
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497. http://doi.org/10.1111/j.1399-3054.1962.tb080 52.x
Muangkaewngam, A., & Te-chato, S. (2018). Morphological and physiological responses of torch ginger [Etlingera elatior (Jack) R.M Smith] to paclobutrazol application. International Journal of Agricultural Technology, 14(4), 559-570. Retrieved from http://www.researchgate.net/
Muangkaewngam, A., & Te-chato, S. (2016). Effect of paclobutrazol on in vitro cultured of torch ginger. Princess of Naradhiwas University Journal, 8(1), 111-116. Retrieved from http://li01.tci-thaijo.org/index.php/pnujr/index
Salari, H., Saninasab, B., Akbari, M., & Rohani, M. A. (2017). Effect of paclobutrazol on adventitious root formation of IBA-treated cuttings of ‘Zard’ and ‘Dakal’olive (Olea europaea L.) cultivars. Asian Journal of Applied Science, 5(4), 692-699. Retrieved from http://ajouronline.com/index.php/AJAS/search/search
Sarkar, S., & Jha, S. (2017). Morpho-histological characterization and direct shoot organogenesis in two types of explants from Bacopa monnieri on unsupplemented basal medium. Plant Cell, Tissue and Organ Culture, 130, 435–441. http://doi.org/10.1007/s11240-017-1231-6
Sharma, S., Kamal, B., Rathi, N., Chauhan, S., Jandon, V., Vats, N., … Arya, S. (2010). In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L) Wettst. African Journal of Biotechnology, 9(49), 8318-8322. Retrieved from http://www.ajol.info/
Sinha, S., Gupta, M., & Chandra, P. (1996). Bioaccumulation and biochemical effects of mercury in the plant Bacopa monnieri (L). Environmental Toxicology and Water Quality, 11, 105–112. http://doi.
org/10.1002/(SICI)1098-2256(1996)11:2<105::AID-TOX5>3.0.CO;2-D
Soumya, P. R., Kumar, P., & Pal, M. (2017). Paclobutrazol: A novel plant growth regulator and multi-stress ameliorant. Indian Journal of Plant Physiology, 22(3), 267-278. http://dio.org/10.1007/s40502-017-0316-x
Subashri, B., & Koilpillai, K. Y. (2014). In vitro regeneration of Bacopa monnieiri (L.) Pennel; A multipurpose medicinal plant. International Journal of Pharmacy and Pharmaceutical Science, 6(4), 559-563. Retrieved from http://www.researchgate.net/
Tiwari, V., Tiwari, K. N., & Singh, B. D. (2001). Comparative studies of cytokinins on in vitro propagation on Bacopa monnieri. Plant Cell, Tissue and Organ Culture, 66, 9-16. Retrieved from http://
www.springer.com/
Tesfahun, W. (2018). A review on: Response of crops to paclobutrazol application. Review Article. Food Science and Technology, 4, 1-9. http://doi.org/10.1080/23311932.2018.1525169
Tropica. (2019). Bacopa ‘Compact’. Retrieved from https://tropica.com/en/plants/plantdetails/Bacopa'Compact'
(044A)/4467
Tsegaw, T., Hammes, S., & Robbertse, J. (2005). Paclobutrazol-induced leaf, stem, and root anatomical modification in potato. Hortscience, 40, 1343-1346. http://doi.org/10.21273/HORTSCI.40.5.1343
Wanderley, C. D. S., Faria, R. T. D., Ventura, M. U., & Vendrame, W. (2014). The effect of plant growth regulators on height control in potted Arundina graminifolia orchids (Growth regulator in Arundina graminifolia). Acta Scientiarum, 36, 489-494. http://doi.org/10.4025/actasciagron.v36i4.18085

Section
Research Articles

##plugins.themes.bootstrap3.article.details##

How to Cite
TAUK, Chanraksmey; CHUADUANGPUI, Pornpimon; KHAWNIAM, Tassanee. Effects of Explants on Plant Regeneration and Concentration of Paclobutrazol on Morphological Responses of Dwarf Water Hyssop (Bacopa monnieri). Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 29, n. 3, p. 56-66, feb. 2021. ISSN 2539-553X. Available at: <https://www.journal.nu.ac.th/NUJST/article/view/Vol-29-No-3-2021-56-66>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.14456/nujst.2021.26.