Antioxidant Activity and Effect of Sa-khan (Piper, Piperaceae) on the Normal Human Proximal Tubular Epithelial Cell Line


Daoyot Daorueang


        The oxidative damage to proximal tubular epithelial cells may lead to renal dysfunction. This research aimed to investigate antioxidant activity of the Sa-khan crude extract (SCE) and evaluated its effect on cell viability of proximal tubular epithelial cells. The total phenolic content of the SCE was determined using Folin-Ciocalteu method. The free radical scavenging activity of the SCE was investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The effect of the SCE on HK-2 cells, the human proximal tubular epithelial cell line, was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The SCE showed the total phenolic content of 17.35 ± 0.40 mg GAE/g extract. In addition, the SCE exhibited scavenging activity on DPPH (IC50 = 1.15+0.071 mg/mL). Moreover, eleven spots of antioxidant compounds were detected when the two-dimensional thin-layer chromatography combined with the DPPH spray technique was performed. Furthermore, the SCE at the concentration of 0.125 mg/mL could promote the proliferation of HK-2 cells over various durations. Together, these results indicate that the SCE may contain compound(s) with potential health benefits.

Keywords: Sa-khan, normal human proximal tubular epithelial cell, antioxidant activity


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Research Articles


How to Cite
DAORUEANG, Daoyot. Antioxidant Activity and Effect of Sa-khan (Piper, Piperaceae) on the Normal Human Proximal Tubular Epithelial Cell Line. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 28, n. 4, p. 29-35, june 2020. ISSN 2539-553X. Available at: <>. Date accessed: 17 jan. 2022. doi: