Adverse Drug Reaction and Preliminary Efficacy of Dioscorea bulbifera Linn. gel on Wound Healing in Thai Patients


Parunkul Tungsukruthai Kusuma Sriyakul Mala Soisamrong Kanyarat Loapiyasakul Piracha Jumpa-Ngern


The lack of access to specialized wound care has resulted in amputations and loss of work productivity and medical cost estimates for chronic wound treatments are high worldwide. The objective of this study was to examine the safety and efficacy of Dioscorea bulbifera Linn. on wound healing in Thai patients before seeking the addition of this drug to the Thai National List of Essential Drugs. This study was conducted in 18 government hospitals from November 2019 to February 2020 with 1,026 OPD and IPD patients. Trained interviewers introduced themselves, explained the purpose of this study to participants, and followed up after using Dioscorea bulbifera Linn gel for 4 weeks at which time measurements were taken using the adverse drug reaction assessment using the Naranjo algorithm, wound characteristics and size. In addition, interviews were used to ascertain patient satisfaction with the drug. The results showed adverse drug reactions in only one patient. Importantly, wound sizes were significantly reduced. Furthermore, 99% of patients would use this drug in the future. These findings provide more evidence to support the traditional use of Dioscorea bulbifera Linn for the treatment of wounds, and this evidence could support its entry into the national list of herbal medicines in Thailand.

Keywords: Dioscorea bulbifera Linn, chronic wound, Wan Phra Chim herb, adverse drug reaction


Ahmed, Z., Chishti, M., Johri, R., Bhagat, A., Gupta, K., & Ram, G. (2009). Antihyperglycemic and antidyslipidemic activity of aqueous extract of Dioscorea bulbifera tubers. Diabetologia Croatica, 38(3), 66-67.
Akbik, D., Ghadiri, M., Chrzanowski, W., & Rohanizadeh, R. (2014). Curcumin as a Wound Healing Agent. Life Sciences, 2014, 116.
Ambiga, S., Narayanan, R., Gowri, D., Sukumar, D., & Madhavan, S. (2007). Evaluation of wound healing activity of flavonoids from ipomoea carnea Jacq. Ancient Science of Life, 26(3), 45-51.
Chadwick, S., Heath, R., & Shah, M. (2012). Abnormal pigmentation within cutaneous scars: A complication of wound healing. Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India, 45(2), 403-411.
Chadwick, S. L., Yip, C., Ferguson, M. W., & Shah, M. (2013). Repigmentation of cutaneous scars depends on original wound type. Journal of Anatomy, 223(1), 74-82.
Chaniad, P., Tewtrakul, S., Sudsai, T., Langyanai, S., & Kaewdana, K. (2020). Anti-inflammatory, wound healing and antioxidant potential of compounds from Dioscorea bulbifera L. bulbils. PLOS ONE, 15(12), e0243632.
Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in pharmacology, 4, 177-177.
Gao, H., Kuroyanagi, M., Wu, L., Kawahara, N., Yasuno, T., & Nakamura, Y. (2002). Antitumor-promoting constituents from Dioscorea bulbifera L. in JB6 mouse epidermal cells. Biological and Pharmaceutical Bulletin, 25(9), 1241-1243.
Ghosh, S., Ahire, M., Patil, S., Jabgunde, A., Bhat Dusane, M., Joshi, B. N., . . . Chopade, B. A. (2012). Antidiabetic Activity of Gnidia glauca and Dioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors. Evid Based Complement Alternat Med, 2012, 929051.
Gopalakrishnan, A., Ram, M., Kumawat, S., Tandan, S., & Kumar, D. (2016). Quercetin accelerated cutaneous wound healing in rats by increasing levels of VEGF and TGF-β1. Indian Journal of Experimental Biology, 54(3), 187-195.
Guo, S. D., & DiPietro, L. A. (2010). Factors Affecting Wound Healing. Journal of dental research, 89, 219-229.
Heath, R., Thomlinson, A., & Shah, M. (2009). Melanocytes and burn wound healing. Burns, 2009, 35.
Hekmatpou, D., Mehrabi, F., Rahzani, K., & Aminiyan, A. (2019). The Effect of Aloe Vera Clinical Trials on Prevention and Healing of Skin Wound: A Systematic Review. Iranian journal of medical sciences, 44(1), 1-9.
Iannone, M., Janowska, A., Dini, V., Tonini, G., Oranges, T., & Romanelli, M. (2019). Itch in Chronic Wounds: Pathophysiology, Impact, and Management. Medicines (Basel, Switzerland), 6(4), 112.
Jayachandran, K. S., Vasanthi, A. H. R., & Gurusamy, N. (2016). Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules. Pharmacognosy magazine, 12(1), S14-S20.
Komori, T. (1997). Glycosides from Dioscorea bulbifera. Toxicon, 35(10), 1531-1536.
Liu, H., Chou, G.-X., Guo, Y.-L., Ji, L.-L., Wang, J.-M., & Wang, Z.-T. (2010). Norclerodane diterpenoids from rhizomes of Dioscorea bulbifera. Phytochemistry, 71(10), 1174-1180.
Liu, H., Chou, G. X., Wu, T., Guo, Y. L., Wang, S. C., Wang, C. H., & Wang, Z. T. (2009). Steroidal sapogenins and glycosides from the rhizomes of Dioscorea bulbifera. Journal of Natural Products, 72(11), 1964-1968.
Mbiantcha, M., Kamanyi, A., Teponno, R. B., Tapondjou, A. L., Watcho, P., & Nguelefack, T. B. (2011). Analgesic and Anti-Inflammatory Properties of Extracts from the Bulbils of Dioscorea bulbifera L. var sativa (Dioscoreaceae) in Mice and Rats. Evid Based Complement Alternat Med, 2011, 912935
Murray, R. D. H., Jorge, Z. D., Khan, N. H., Shahjahan, M., & Quaisuddin, M. (1984). Diosbulbin d and 8-epidiosbulbin e acetate, norclerodane diterpenoids from Dioscorea bulbifera tubers. Phytochemistry, 23(3), 623-625.
Panduraju, T., Bitra, V. R., Vemula, S. K., & Reddy, P. R. V. (2010). Wound healing activity of Dioscorea bulbifera Linn. Journal of Pharmacy Research, 3(12), 3138-3139.
Payyappallimana, U. (2010). Role of Traditional Medicine in Primary Health Care : An Overview of Perspectives and Challenges. Yokohama Journal of Social Sciences, 14, 57-77.
Pepper, F. J. (1954). The epithelial repair of skin wounds in the guinea-pig with special reference to the participation of melanocytes. Journal of Morphology, 95(3), 471-499.
Wang, J.-M., Ji, L.-L., Branford-White, C. J., Wang, Z.-Y., Shen, K.-K., Liu, H., & Wang, Z.-T. (2012). Antitumor activity of Dioscorea bulbifera L. rhizome in vivo. Fitoterapia, 83(2), 388-394.
Wang, J., Ji, L., Liu, H., & Wang, Z. (2010). Study of the hepatotoxicity induced by Dioscorea bulbifera L. rhizome in mice. BioScience Trends, 4(2), 79-85.

Research Articles


How to Cite
TUNGSUKRUTHAI, Parunkul et al. Adverse Drug Reaction and Preliminary Efficacy of Dioscorea bulbifera Linn. gel on Wound Healing in Thai Patients. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 30, n. 4, p. 66-74, sep. 2022. ISSN 2539-553X. Available at: <>. Date accessed: 17 june 2024. doi: