Effects of Different Nitrogen Forms on Growth, Phenolic Content, and Antioxidant Activity in Hedychium speciosum and H. coronarium

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Janjira Muenrew Ratchuporn Suksathan Ratchadawan Puangpradab Apinya Rachkeeree Kuttiga Kantadoung

Abstract

        Nitrogen (N) is the most important nutrient element for plant growth and the synthesis of many secondary metabolites.  Plants typically obtain inorganic N in form of NH4+ and NO3-. Two Hedychium species were grown in nutrient solutions modified from Smart and Barko (1985). The experiment consists of three N forms; NH4+, NH4+NO3-, NO3- and control (no N form)  with the same concentration of nitrogen (500 µM) for 60 days. Destructive sampling was done and morphology was recorded. Total phenolic content (TPC) was evaluated by Folin-Ciocalteu method while the antioxidant activity was evaluated by DPPH and ABTS radical scavenging activity. Different nitrogen forms have significantly (p ≤0.05) affected plant height, root numbers, root number and total biomass between two Hedychium species. H. speciosum had height new shoot, leaf number, root number and total biomass in NH4+ supply. H. coronarium had height, leaf number, root number and total biomass in NH4+NO3- supply. Total phenolic contents both species were increased by NH4+ supply. H. speciosum were accumulate total phenolic content in the leaves 21.55 mg.g-1 GAE was lower than in H. coronarium  43.49 mg.g-1GAE, but in stem of the  H. speciosum were accumulate total phenolic content 25.08 mg.g-1GAE was lower than in H. coronarium 20.66 mg.g-1GAE.  NH4+ supply increased antioxidant activity with DPPH radical scavenging activity and ABTS radical scavenging activity.


Keywords: Phenolic content, Antioxidant activity, Nitrogen forms, Hedychium species, Growth, Morphology

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Section
Research Articles

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How to Cite
MUENREW, Janjira et al. Effects of Different Nitrogen Forms on Growth, Phenolic Content, and Antioxidant Activity in Hedychium speciosum and H. coronarium. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 29, n. 2, p. 33-42, sep. 2020. ISSN 2539-553X. Available at: <http://www.journal.nu.ac.th/NUJST/article/view/Vol-29-No-2-2021-33-42>. Date accessed: 03 dec. 2021. doi: https://doi.org/10.14456/nujst.2021.14.