The Development of Blood Stain Removal Solution from Spinach in Longer Storage at Room Temperature for Sanitation Work in Hospitals


Nalin-on Nuiplot


        The cleanliness in hospital is very essential for preventing of germ spreading especially from the secretion contaminated on clothes of patients such as saliva, lymph, and blood. Nowadays, two widely-used methods for the blood stain remover on patients’ clothes are Hypochlorite using which contains the irritated chemical components, and Ozone disinfector which is expensive and has a risk of ozone leaking causing accumulation and toxic to the body. However, a previous study had used the extract from spinach to test the blood stain remover. The spinach contains a large amount of oxalate which can be precipitated with metal ion (Ferrous ion in blood) and then be removed easily. Nevertheless, there are limitations in terms of time and place in storing the extract. This research therefore aims to develop blood stain removal solution from spinach by testing against detergent N70 to be conveniently stored and prolong the storage time in room temperature. The spinach samples were available from 3 areas in Thung Yai District, Nakhon Si Thammarat Province, Thailand. The solution from spinach was mixed with the solution of 500 g Chloride salt in 3 liters of water and 1 kg of the anionic surfactant substance; Sodium Laurylether sulfate (SLES) or Texapon N70 or N70. The mixed solution was tested with blood stained clothes which were donated from Ban Khlong Priang Subdistrict Health Promotion Hospital, Thung Yai District, Nakhon Si Thammarat Province, Thailand. The results showed that the developed blood stain remover from spinach has a storage period of up to 6 months; from normal: only 1 day at room temperature or 7 days at 4 degrees Celsius. The detergents also do not contain phosphate chemical resulting in being a non-toxic stain remover solution. Moreover, it is cost only 70 Bath per 1 liter then it can reduce the cost of buying stain remover solution about 3 times. As a result, it will help reduce the cost of purchasing blood stain remover which is a deeply stain and is often found in households and various medical institutions.

Keywords: Stain remover, Sanitary service, Blood stain, Oxalate, Spinach


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Research Articles


How to Cite
NUIPLOT, Nalin-on. The Development of Blood Stain Removal Solution from Spinach in Longer Storage at Room Temperature for Sanitation Work in Hospitals. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 29, n. 2, p. 1-10, sep. 2020. ISSN 2539-553X. Available at: <>. Date accessed: 03 dec. 2021. doi: