Efficiency of Using Trichoderma, Mucor and Aspergillus Antimicrobial Pellets to Rhizophora mucronata Poir. Planting at Abandoned Shrimp Farm in Khanom District, Nakhon Si Thammarat Province, Thailand

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Sukhan Rattanaloeadnusorn

Abstract

     Rhizophora mucronata Poir planting was non using antimicrobial pellets in spreading pathogens of genus Fusarium, Polyporus at abandoned shrimp farm area, Khanom District, Nakhon Si Thammarat Province, Thailand. The plants were short with 33% survival rate, low macronutrient, micronutrients and supplemented nutrients. Only 9 isolates of fungal biodiversity were found. When Trichoderma antimicrobial pellets were used to induce 2-3-folds of growth, 95 % of R. mucronata plants survived. This aimed to compare the height growth, survival rate, physical and biological properties before and after using the Trichoderma, Mucor and Aspergillus multi-antimicrobial pellets. However, using the Trichoderma, Mucor and Aspergillus multi-antimicrobial pellets for R. mucronata planting in spreading pathogens of Fusarium, Polyporus area, the highest growth was 4.30 folds and 99% of the plants survived. Furthermore, using these pellets in non pathogens, the growth was 4.02 folds and 99% of the plants survived, while the growth was 2.64 folds and 88% of the plants survived in the spreading area of these pellets. On the other hand, in the controlled area the growth was 0.42 folds and 88% of the plants survived. When the Trichoderma, Mucor and Aspergillus multi-antimicrobial secreted 1-aminocyclopropane-1-carboxylate (ACC) extraenzymes, ethylene synthesis decreased. When this ethylene in the plant reduced, the antimicrobial secreted. This induced IAA hormone to speed up the plants growth, dissolved phosphorus and fixed nitrogen. Macronutrients, micronutrients and supplemented nutrients increased in sediment soil between 45 to 2000%, while heavy metals reduced between 80 to 100%. The coefficients were more accurate than 95.5.  So government should develop R. mucronata or other mangrove plants in spread or non spread pathogens area by using Trichoderma, Mucor and Aspergillus multi-antimicrobial pellets in abandoned shrimp farm. Consequently, the area will be restored and returned to natural balance within 5 years.


 Keywords: Trichoderma, multi-Antimicrobial Pellets, Rhizophora mucronata, Survival, Growth, Mangrove forest

References

Ahemad, M., & Malik A. (2011). Bioaccumulation of heavy metals by zinc resistant bacteria isolated from agricultural soils irrigated with wastewater. Bacteriology Journal, 2(1), 12-21.
Aksornkoae, S., & Khemnark, C. (1994). Nutrient cycling in mangrove forest of Thailand Proc. As. In Symp. Mangrov-Res & Manag, 113, 83-94.
Behera, B. C. H., Yadav, S. K., Singh, R., Mishr, A., Set hi, B. K., Dutta, S. K., & Thatoi, H. N. (2017). Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta. Odisha. India Journal of Genetic Engineering and Biotechnology, 15(1), 169-178.
Bryant, D., Nielsen, D., & Tangley, L. (1997). The last frontier forests: Ecosystems & Economics on the edge.WRI, World Conservation Monitoring Centre and the World Wildlife Fund. Washington, D.C.: World Resources Institute, Forest Frontiers Initiative 39.
Figueiredo, M. V. B., Burity, H. A., Martınez, C. R. & Chanway, C. P. (2008). Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici. Applied Soil Ecology, 40, 182-188.
Glick, B. R., Cheng, Z., Czarny, J., Cheng, Z., & Duan, J. (2007). Promotion of plant growth by ACC deaminase-producing soil bacteria. European Journal of Plant Pathology, 119, 329-339.
Govindasamy, V., Senthilkumar, M., Mageshwaran, V., & Annapurna, K. (2008). Detection and Characterization of ACC Deaminase in Plant Growth Promoting Rhizobacteria. Journal of Plant Biochemistry and Biotechnology, 18(1), 71–76.
Harris, R. F., & Sommers, L. E. (1968). Plate-Dilution Frequency Technique for Assay of Microbial Ecology. Applied and Environmental Microbiology, 16(2), 330-334.
Intana, W., Chamswang, C., Intanoo, W., Hongprayoo, C., & Sivasithamparam, K. (2003). Use of mutantstrain for improved efficacy of Trichodrema for controling cucumber damping-off. Thai Journal of Agricultural Science, 36(3), 45-50.
Lavakush, Y., Janardan, V., Jay, P., Jaiswal, D. K.., & Kumar, A. (2014). Evaluation of PGPR and different
concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa). Ecological Engineering, 62, 123-128.
Preedanon, S., Klaysuban, A., Suetrong, S., Promchoo, W., Gundool, W., Sangtiean, T., & Sakayaroj, J. (2017). Helicascus mangrovei sp. nov., a new intertidal mangrove fungus from Thailand. Mycoscience, 58(3), 174-180.
Rattanaloeadnusorn, S. (2017). Inoculants Fungal Trichoderma, Mucor and Bacillus for Community Development based on sufficiency economy philosophy. International Journal of Geomate, 13(40), 16-23. https://doi.org/10.21660/2017.40.2517
Rattanaloeadnusorn, S., Sirikhae, P., & Atchanut, R. (2014). Antagonistic Fungal Pellets for Community Development based Sufficiency Economy Philosophy. Retrieved from https://www.science-community.org/en/node/85900
Rattanaloeadnusorn, S., Thitaya, S., Sujaya, R., & Sirikhae, P. (2012). Biofertilizer from Stock Fungus and Natural Material for Suf ficiency Economy Philosophy Community Development.International Symposium on Local Wisdom and Improving Quality of Life, 8-11 August 2012 (pp. 49-56). Chai mai: Thailand.
Sakayaroj, J., Preedanon, S., Suetrong, S., Klaysuban, A., Jones, E. B. G., & Hattori, T. (2012). Molecular characterization of basidiomycetes associated with the decayed mangrove tree Xylocarpus granatum in Thailand. Fungal Diversity, 56(1), 145-156.
Sofia, G. (1998). Growth models and their use in ecological modelling: an application to a fish population. Ecological Modelling, 113, 83-94.
Vaxevanidou, K. (2008). Removal of heavy metals and arsenic from contaminated soils using bioremediation and chelant extraction techniques. Chemosphere, 70, 1329-1337.
Wijarn, M. (2009). Growth and Litter Production of 7-10 Years Old Rhizophora mucronata Lamk. Planted on Abandoned Shrimp Farm Area at Donsak, Surat Thani. Province Journal of Forest Management, 3(6), 35-45.

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How to Cite
RATTANALOEADNUSORN, Sukhan. Efficiency of Using Trichoderma, Mucor and Aspergillus Antimicrobial Pellets to Rhizophora mucronata Poir. Planting at Abandoned Shrimp Farm in Khanom District, Nakhon Si Thammarat Province, Thailand. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 27, n. 4, p. 10-19, oct. 2019. ISSN 2539-553X. Available at: <http://www.journal.nu.ac.th/NUJST/article/view/Vol-27-No-4-2019-10-19>. Date accessed: 22 nov. 2019. doi: https://doi.org/10.14456/nujst.2019.32.