Monitoring of heavy metal contamination in aquatic organism by applying Metallothionein biomarker and its situation in Thailand
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Published
Mar 23, 2016
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Abstract
Metallothionenein (MT) is produced in the liver after the organism exposed to heavy metals. MT is involved in homeostasis and detoxification process of heavy metals. Thus, MT has the potential to be applied as a bioindicator for heavy metal exposure of the organisms especially the aquatic ones. MT is characterized based on a high content of sulphur, cadmium and zinc. In present, the assessment on heavy metal contamination can be used to detect MT both directly and indirectly, i.e. (1) direct methods: the measurement of MT content by the difference pulse polarography (DPP) and spectrophotometry and (2) indirect methods: the measurement of MT content in each organ of aquatic organism by using antibody technique such as Enzyme-linked immunosorbent assay (ELISA) or western blot. The measurement on expression level of gene controlling MT production by many biomolecular techniques allowing to evaluate heavy metal exposure condition of the organism. In Thailand, the study on MT and its application have been inadequate. There were two cases of MT application as biomarker found: Ang sila (Chonburi province) and Map ta put (Rayong province). Thus it should be more studied for developing and extending its application.
Keywords: Metallothionein, Biomarker, Heavy metal, Aquatic organisms
References
Adam, V., Fabrik, I., Eckchlager, T., Stiborova, M., Trnkova, L., & Kizek, R. (2010). Vertebrate metallthioneins as target molecules for analytical technique. Trends in Analytical Chemistry, 29(5), 409-418.
Adhikari, S., Ghosh, L., Giri, B., & Ayyappan, S. (2009). Distributions of metals in the food web of fishponds of Kolleru Lake, India. Ecotoxicology and Environmental Safety, 72, 1242-1248.
Alvarado, N. E., Quesada, I., Hylland, K., Marigomez, I., & Sato, M. (2006). Quantitative changes in metallothionein in target cell-types in the gills of turbot (Scopthalmus maximus) exposed to Cd, Cu, Zn, and after a depuration treatment. Aquatic toxicology, 77, 64-77.
Amiard, J. C., Amiard-Triquet, C., Barka, S., Pellerin, J., & Rainbow, P. S. (2006). Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquatic toxicology, 76, 160-170.
Andrew, G. K. (2000). Regulation of metallothionein gene expression by oxidative stress and metals ions. Biochemistry and Pharmacology, 59, 95-104.
Barnette, P., Panutrakul. S., Nanthanawat, P., Pattarabuddha, N., Maharachpong, N., Mokkongpai, P., & Celender, M. C. (2013). Assessments of heavy metals and organic hydrocarbons exposure in selected marine animals from coastal industrial area at Map Ta Phut, Rayoung Province. Burapha University: Chonburi.
Capasso, C., Carginale, V., Crescenzi, O., Maro, D. D., Parisi, E., Spadaccint, R., & Temussi, P. A. (2003). Solution structure of MT nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps. Structure, 11, 435-443.
Castro-González, M. I., & Méndez –Armenta, M. (2008). Heavy metals: Implications associated to fish consumption. Ecotoxicology and Environmental Safety, 26, 263-271.
Campenhout, K. V., Infante, H. G., Hoff, P. T., Moens, L., Goemans, G., Belpaire, C., ... Bervoets. (2004). Cytosolic distribution of Cd, Cu, and Zn and metallothionein levels in relation to physiological changes in gibel carp (Carasius auratus gibelio). Ecotoxicology and Environmental Safety, 73, 296-305.
Chinda, P. (2003). Metallothionein as a biomarker for mercury contamination in mussel, Perna viridis. Chulalongkorn University: Bangkok.
Cho, Y. S., Lee, S. Y., Kim, K. Y., & Nam, Y. K. (2009). Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis. Comparative Biochemistry and Physiology: Biochemistry Molecular Biology, 153(4), 317-326.
Filipovic, V., & Raspor, B. (2003). Metallothionein and metal levels in cytosol of liver, kidney and brain in relation to growth parameters of Mullus surmuletus and Liza aurata from the Eastern Adriatic Sea. Water Research, 37, 3253-3262.
Hogstrand, C., & Haux, C. (1991). Binding and detoxification of heavy metals in Lower vertebrates with references to metallothionein. Comparative Biochemical and Physiological, 113C(2), 109-115.
Honda, R. T., Araujo, R. M., Horta, B. B., Val, A. L., & Demasi, M. (2005). One-step purification of metallothionein extracted from two different sources. Journal of Chromatography, 100C(1/2), 137-141.
Huggett, R. J., Kimerle, R. A., Mehrle, P. M., & Bergman, H. L. (1992). Biomarkers Biochmical, physiological, and histological marker of anthropogenic stress. Lewis: USA.
Kagi, J. H. R., & Kojima. (1979). Nomenclature of metallothionein: a proposal. Experientia, Supplementum, 34, 141-142.
Kessabi, K., Navarro, A., Casado, M., Said, K., Messaoudi, I., & Pina, B. (2010). Evaluation of environmental impact on natural population of the Mediterrenean killifish Aphanius fasciatus by quantitative RNA biomarker. Marine Environmental Research, 70, 327-333.
Kim, J., Wang, S., Kim, I., Ki, J., Raisuddin, S., Lee, J., & Han, K. (2008). Cloning of river pufferfish (Takifugu obscurrus) metallothionein cDNA and study of its induction profile in cadmium-exposed fish. Chemosphere, 71, 1251-1259
Klaassen, C. D., Lui, J., & Choudhuri, S. (1999). Metallothionein: An intracellular protein to protect against cadmium toxicity. Annual Review in Phamacology, 39, 267-294.
Klaverkamp, J. F., Wautier, K., & Baron, C. L. (2000). A modified mercury saturation assay for measuring metallothionein. Aquaculture Toxicology, 50, 13–25.
Knapen, D., Reynders, H., Bervoets, L., Verheyen, E., & Blust, R. (2007). Metallothionein gene and protein expression as a biomarker for metal pollution in natural gudgeon populations. Aquatic Toxicology, 82, 163-172.
Kovarova, J., Kizek, A. V., Harustiakova, D., Celechovska, O., & Svobodova, Z. (2005). Effect of cadmium chloride on metallothionein levels in Carp. Sensor, 9, 4789-4803.
Krizkova, S., Zitka, O., Adam, V., Beklova, M., Horna, A., Svobodova, Z., ... Kizek, R . (2007). Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues. Czech Journal of Animal Science, 52(5), 143-148.
Langston, W. J., Chesman, B. S., Burt, G. R., Pope, N. D., & McEvoy, J. (2002). Metallothionein in liver of eels Anguilla Anguilla from the Thames Estuary: an indicator of environmental quality? Marine Environmental Research, 53, 263-293.
Linde, A. R., Sanchez-Galan, S., Valles-Mota, P., & Garcia-Vazquez, E. (2001). Metallothionein as bioindicator of freshwater metal pollution: European eel and Brown trout. Ecotoxicology and Environmental safety, 49, 60-66.
Lacorn, M., Lahrssen, A., Rotzoll, N., Simat, T. J., & Steinhart, H. (2001). Quantification of metallothionein isoforms in fish liver and its implications for biomornitoring. Environmental Toxicology and Chemistry, 20(1), 140-145.
Livingstone, D. R. (1993). Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. Journal of Chemical Technology and Biotechnology, 57, 195-211.
Monserrat, J. M., Martinez, P. E., Geracitano, L. A., Amado, L. L., Martins, C. M. G., Pinho, G. L. L., ... Bianchini, A. (2007). Pollution biomarkers in estuarine animals: Critical review and new perspectives. Comparative Biochemistry and Physiology Part C, 146, 221-234.
Montaser, M., Mahfouz, M. E., El-Shazly, S. A. M., Abdel-Rahman, G. H., & Bakry, S. (2010). Toxicity of heavy metals on fish at Jeddah coast KSA: Metallothionein expression as a biomarker and histopathological study on liver and gills. World Journal of fish and marine Science, 2(3), 174-185.
Nordberg, M. (1998). Metallothioneins: historical review and state of knowledge. Talanta, 46, 243-254.
Olsvik, P. A., Hindar, K., Zachariassen, K. E., & Andersen, R. A. (2001). Brown trout, Salmo trutta, metallothioneins as biomarkers for metal exposure in two Norwegian. Biomarkers, 6(4), 274-288.
Oost, V. D., Beyer, R., Vermeulen, J., & Nico, P. E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13, 57-149.
Peakall, D. A. (1994). The role of biomarkers in environmental assessment. Ecotoxicology, 3, 157-160.
Ramo, J. D., Torreblanca, A., Martinez, M., Pastor, A., & Diaz-Mayans, J. (1995). Quantification of cadmium-induced metallothionein in crustaceans by the silver-saturation method. Comparative Biochemistry and physiology, 141(C), 303-314.
Roesijadi, G. (1992). Metallothioneins in metal regulation and toxicity in aquatic animals. Aquatic Toxicology, 22, 81-114.
Rotchell, J. M., Clarke, K. R., Newton, L. C., & Bird, D. J. (2001). Hepatic metallothionein as a biomarker for metal contamination: age effects and seasonal variation in European flounders (Pleuronectes flesus) from the seven estuary and Bristol channel. Marine Environmental Research, 52, 151-171.
Sevcikova, M., Modra, H., Kruzikova, K., Zitka, O., Hynek, D., Adam, V., ... Svobodova, Z. (2013). Effect of Metals on Metallothionein Content in Fish from Skalka and Želivka Reservoirs. International Journal of Electrochemical Science, 8, 1650 – 1663.
Siscar, R., Koenig, S., Torreblanca, A., & Solé, M. (2013). The role of Metallothionein and selenium in metal detoxification in the liver of deep-sea fish from the NW Mediterranean Sea. Science of the Total Environment, 1(466-467), 898–905.
Stegeman, J. J., Brouwer, M., Digiulio, R. T., Forlin, L., Fowler, B. M., Sanders, B. M., & Van, P. A. (1992). Molecular responses to environmental contamination: enzyme and protein systems as indicators of contaminant exposure and effect. Lewis: USA.
Thanomsit, C., Nantanawat, P. Wassmur, B., Grans, J., Calander, M., & Barnette, P. (2013). Characterization of metallothionein from Asian sea bass (Lates calcarifer, Bloch) and application as a biomarker for heavy metal exposure in Thailand. Asian journal of water, Environment and pollution, 10(4), 53-64.
Vergani, L., Grattarola, M., Dondero, F., & Viarengo, A. (2003). Expression, purification and characterization of metallothionein-A from rainbow trout. Protein Expression and Purification, 27(2), 338-345.
Viarengo, A., Burlando, B., Cerratto, B., & Panfoli, I. (2000). Antioxidant role of metallothioneins: a comparative overview. Cellular and Molecular Biology, 46, 407-417.
Viarengo, A., Burlando, B., Evangelisti, V., Mozzone, S., & Dondero, F. (2001). Sensitivity and specificity of metallothionein as a biomarker for aquatic environment biomornitoring. Biomarker in Marine Organism: A Practical Approach, 29-43.
Walker, C. H., Hopkin, S. P., Sibly, R. M., & Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis: USA.
Wu, S. M., Shih, M., & Ho, Y. (2007). Toxicological stress response and cadmium distribution in hybrid tilapia (Oreochromis sp.) upon cadmium exposure. Comparative Biochemistry and Physiology, Part C, 145, 218-226.
Yudkovski, Y., Rogowska-Wrzesinska, A., Yankelevich, I., Shefer, E., Herut, B., & Tom, M. (2008). Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Marine Environmental Research, 65, 427-436.
Adhikari, S., Ghosh, L., Giri, B., & Ayyappan, S. (2009). Distributions of metals in the food web of fishponds of Kolleru Lake, India. Ecotoxicology and Environmental Safety, 72, 1242-1248.
Alvarado, N. E., Quesada, I., Hylland, K., Marigomez, I., & Sato, M. (2006). Quantitative changes in metallothionein in target cell-types in the gills of turbot (Scopthalmus maximus) exposed to Cd, Cu, Zn, and after a depuration treatment. Aquatic toxicology, 77, 64-77.
Amiard, J. C., Amiard-Triquet, C., Barka, S., Pellerin, J., & Rainbow, P. S. (2006). Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquatic toxicology, 76, 160-170.
Andrew, G. K. (2000). Regulation of metallothionein gene expression by oxidative stress and metals ions. Biochemistry and Pharmacology, 59, 95-104.
Barnette, P., Panutrakul. S., Nanthanawat, P., Pattarabuddha, N., Maharachpong, N., Mokkongpai, P., & Celender, M. C. (2013). Assessments of heavy metals and organic hydrocarbons exposure in selected marine animals from coastal industrial area at Map Ta Phut, Rayoung Province. Burapha University: Chonburi.
Capasso, C., Carginale, V., Crescenzi, O., Maro, D. D., Parisi, E., Spadaccint, R., & Temussi, P. A. (2003). Solution structure of MT nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps. Structure, 11, 435-443.
Castro-González, M. I., & Méndez –Armenta, M. (2008). Heavy metals: Implications associated to fish consumption. Ecotoxicology and Environmental Safety, 26, 263-271.
Campenhout, K. V., Infante, H. G., Hoff, P. T., Moens, L., Goemans, G., Belpaire, C., ... Bervoets. (2004). Cytosolic distribution of Cd, Cu, and Zn and metallothionein levels in relation to physiological changes in gibel carp (Carasius auratus gibelio). Ecotoxicology and Environmental Safety, 73, 296-305.
Chinda, P. (2003). Metallothionein as a biomarker for mercury contamination in mussel, Perna viridis. Chulalongkorn University: Bangkok.
Cho, Y. S., Lee, S. Y., Kim, K. Y., & Nam, Y. K. (2009). Two metallothionein genes from mud loach Misgurnus mizolepis (Teleostei; Cypriniformes): gene structure, genomic organization, and mRNA expression analysis. Comparative Biochemistry and Physiology: Biochemistry Molecular Biology, 153(4), 317-326.
Filipovic, V., & Raspor, B. (2003). Metallothionein and metal levels in cytosol of liver, kidney and brain in relation to growth parameters of Mullus surmuletus and Liza aurata from the Eastern Adriatic Sea. Water Research, 37, 3253-3262.
Hogstrand, C., & Haux, C. (1991). Binding and detoxification of heavy metals in Lower vertebrates with references to metallothionein. Comparative Biochemical and Physiological, 113C(2), 109-115.
Honda, R. T., Araujo, R. M., Horta, B. B., Val, A. L., & Demasi, M. (2005). One-step purification of metallothionein extracted from two different sources. Journal of Chromatography, 100C(1/2), 137-141.
Huggett, R. J., Kimerle, R. A., Mehrle, P. M., & Bergman, H. L. (1992). Biomarkers Biochmical, physiological, and histological marker of anthropogenic stress. Lewis: USA.
Kagi, J. H. R., & Kojima. (1979). Nomenclature of metallothionein: a proposal. Experientia, Supplementum, 34, 141-142.
Kessabi, K., Navarro, A., Casado, M., Said, K., Messaoudi, I., & Pina, B. (2010). Evaluation of environmental impact on natural population of the Mediterrenean killifish Aphanius fasciatus by quantitative RNA biomarker. Marine Environmental Research, 70, 327-333.
Kim, J., Wang, S., Kim, I., Ki, J., Raisuddin, S., Lee, J., & Han, K. (2008). Cloning of river pufferfish (Takifugu obscurrus) metallothionein cDNA and study of its induction profile in cadmium-exposed fish. Chemosphere, 71, 1251-1259
Klaassen, C. D., Lui, J., & Choudhuri, S. (1999). Metallothionein: An intracellular protein to protect against cadmium toxicity. Annual Review in Phamacology, 39, 267-294.
Klaverkamp, J. F., Wautier, K., & Baron, C. L. (2000). A modified mercury saturation assay for measuring metallothionein. Aquaculture Toxicology, 50, 13–25.
Knapen, D., Reynders, H., Bervoets, L., Verheyen, E., & Blust, R. (2007). Metallothionein gene and protein expression as a biomarker for metal pollution in natural gudgeon populations. Aquatic Toxicology, 82, 163-172.
Kovarova, J., Kizek, A. V., Harustiakova, D., Celechovska, O., & Svobodova, Z. (2005). Effect of cadmium chloride on metallothionein levels in Carp. Sensor, 9, 4789-4803.
Krizkova, S., Zitka, O., Adam, V., Beklova, M., Horna, A., Svobodova, Z., ... Kizek, R . (2007). Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues. Czech Journal of Animal Science, 52(5), 143-148.
Langston, W. J., Chesman, B. S., Burt, G. R., Pope, N. D., & McEvoy, J. (2002). Metallothionein in liver of eels Anguilla Anguilla from the Thames Estuary: an indicator of environmental quality? Marine Environmental Research, 53, 263-293.
Linde, A. R., Sanchez-Galan, S., Valles-Mota, P., & Garcia-Vazquez, E. (2001). Metallothionein as bioindicator of freshwater metal pollution: European eel and Brown trout. Ecotoxicology and Environmental safety, 49, 60-66.
Lacorn, M., Lahrssen, A., Rotzoll, N., Simat, T. J., & Steinhart, H. (2001). Quantification of metallothionein isoforms in fish liver and its implications for biomornitoring. Environmental Toxicology and Chemistry, 20(1), 140-145.
Livingstone, D. R. (1993). Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. Journal of Chemical Technology and Biotechnology, 57, 195-211.
Monserrat, J. M., Martinez, P. E., Geracitano, L. A., Amado, L. L., Martins, C. M. G., Pinho, G. L. L., ... Bianchini, A. (2007). Pollution biomarkers in estuarine animals: Critical review and new perspectives. Comparative Biochemistry and Physiology Part C, 146, 221-234.
Montaser, M., Mahfouz, M. E., El-Shazly, S. A. M., Abdel-Rahman, G. H., & Bakry, S. (2010). Toxicity of heavy metals on fish at Jeddah coast KSA: Metallothionein expression as a biomarker and histopathological study on liver and gills. World Journal of fish and marine Science, 2(3), 174-185.
Nordberg, M. (1998). Metallothioneins: historical review and state of knowledge. Talanta, 46, 243-254.
Olsvik, P. A., Hindar, K., Zachariassen, K. E., & Andersen, R. A. (2001). Brown trout, Salmo trutta, metallothioneins as biomarkers for metal exposure in two Norwegian. Biomarkers, 6(4), 274-288.
Oost, V. D., Beyer, R., Vermeulen, J., & Nico, P. E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13, 57-149.
Peakall, D. A. (1994). The role of biomarkers in environmental assessment. Ecotoxicology, 3, 157-160.
Ramo, J. D., Torreblanca, A., Martinez, M., Pastor, A., & Diaz-Mayans, J. (1995). Quantification of cadmium-induced metallothionein in crustaceans by the silver-saturation method. Comparative Biochemistry and physiology, 141(C), 303-314.
Roesijadi, G. (1992). Metallothioneins in metal regulation and toxicity in aquatic animals. Aquatic Toxicology, 22, 81-114.
Rotchell, J. M., Clarke, K. R., Newton, L. C., & Bird, D. J. (2001). Hepatic metallothionein as a biomarker for metal contamination: age effects and seasonal variation in European flounders (Pleuronectes flesus) from the seven estuary and Bristol channel. Marine Environmental Research, 52, 151-171.
Sevcikova, M., Modra, H., Kruzikova, K., Zitka, O., Hynek, D., Adam, V., ... Svobodova, Z. (2013). Effect of Metals on Metallothionein Content in Fish from Skalka and Želivka Reservoirs. International Journal of Electrochemical Science, 8, 1650 – 1663.
Siscar, R., Koenig, S., Torreblanca, A., & Solé, M. (2013). The role of Metallothionein and selenium in metal detoxification in the liver of deep-sea fish from the NW Mediterranean Sea. Science of the Total Environment, 1(466-467), 898–905.
Stegeman, J. J., Brouwer, M., Digiulio, R. T., Forlin, L., Fowler, B. M., Sanders, B. M., & Van, P. A. (1992). Molecular responses to environmental contamination: enzyme and protein systems as indicators of contaminant exposure and effect. Lewis: USA.
Thanomsit, C., Nantanawat, P. Wassmur, B., Grans, J., Calander, M., & Barnette, P. (2013). Characterization of metallothionein from Asian sea bass (Lates calcarifer, Bloch) and application as a biomarker for heavy metal exposure in Thailand. Asian journal of water, Environment and pollution, 10(4), 53-64.
Vergani, L., Grattarola, M., Dondero, F., & Viarengo, A. (2003). Expression, purification and characterization of metallothionein-A from rainbow trout. Protein Expression and Purification, 27(2), 338-345.
Viarengo, A., Burlando, B., Cerratto, B., & Panfoli, I. (2000). Antioxidant role of metallothioneins: a comparative overview. Cellular and Molecular Biology, 46, 407-417.
Viarengo, A., Burlando, B., Evangelisti, V., Mozzone, S., & Dondero, F. (2001). Sensitivity and specificity of metallothionein as a biomarker for aquatic environment biomornitoring. Biomarker in Marine Organism: A Practical Approach, 29-43.
Walker, C. H., Hopkin, S. P., Sibly, R. M., & Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis: USA.
Wu, S. M., Shih, M., & Ho, Y. (2007). Toxicological stress response and cadmium distribution in hybrid tilapia (Oreochromis sp.) upon cadmium exposure. Comparative Biochemistry and Physiology, Part C, 145, 218-226.
Yudkovski, Y., Rogowska-Wrzesinska, A., Yankelevich, I., Shefer, E., Herut, B., & Tom, M. (2008). Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Marine Environmental Research, 65, 427-436.
Keywords
Metallothionein, Biomarker, Heavy metal, Aquatic organisms
Section
Review Articles
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How to Cite
THANOMSIT, Chutima.
Monitoring of heavy metal contamination in aquatic organism by applying Metallothionein biomarker and its situation in Thailand.
Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 24, n. 1, p. 1-12, mar. 2016.
ISSN 2539-553X.
Available at: <https://www.journal.nu.ac.th/NUJST/article/view/1242>. Date accessed: 26 apr. 2024.