TY - JOUR AU - Suphrom, Nungruthai AU - Sonyot, Witsanu AU - Insumrong, Kamonluk AU - Sawangsup, Pimtawan AU - Sutamuang, Phitchaporn AU - Ingkaninan, Kornkanok PY - 2017 TI - GC-MS analysis and in vitro anti-androgenic activity of Kaempferia rotunda Linn extract JF - Naresuan University Journal: Science and Technology (NUJST); Vol 25 No 4 (2017): October - December 2017 KW - Kaempferia rotunda; Anti-androgen; 5α-Reductase; GC-MS; Sesquiterpenes N2 -      Testosterone is converted to a more potent androgen, dihydrotestosterone (DHT) by 5α-reductase and causes various hormonal actions. Anti-androgen can be used for the treatment of several disorders related to male sex hormone. In this study, the in vitro anti-androgenic activity and chemical components of hexane, dicholomethane and ethanolic extracts of Kaempferia rotunda Linn. rhizome were investigated. The various concentrations of extracts were tested on anti-androgenic activity by acting against the conversion of testosterone. The hexane extract was the most potent (IC 50 = 0.43 ± 0.10 mg/mL) and showed the IC 50 value in the same range to that of positive control; ethinylestradiol (IC 50 = 0.26 ± 0.02 mg/mL). The different chemical profiles of all extracts studied were observed by GC-MS. The results indicated that most of volatile components including mono- and sesquiterpenes were present in hexane extract. Further studies on isolation and identification of active components are needed. This plant might be a new source for anti-androgens. Keywords: Kaempferia rotunda , Anti-androgen, 5α-Reductase, GC-MS, Sesquiterpenes UR - https://www.journal.nu.ac.th/NUJST/article/view/1482