Prevalence and Antimicrobial Resistance of Salmonella in Minced Pork from Retail Shops around the University of Phayao, Thailand
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Abstract
Salmonella is a common pathogen causing food-borne diseases in humans when contaminated food is consumed. As a food commonly consumed in Northern Thailand, raw pork consumption is a common source of contamination. However, there is limited information of the extent and prevalence of Salmonella contamination in this region. This current study was a preliminary investigation of the prevalence of Salmonella in minced pork from retail shops around the University of Phayao, Thailand, and an analysis of the antimicrobial resistance of the Salmonella. A total of 35 minced pork samples were randomly collected from retail shops in the area, and Salmonella isolation and identification were performed following ISO 6579. Serogrouping was examined by slide agglutination, and antimicrobial susceptibility testing was performed by the disk diffusion method, based on recommendations of Clinical and Laboratory Standard Institute (CLSI), 2017. Our findings were that a total of 42 Salmonella isolates were isolated from 35 samples. The most frequently isolated serogroup was Group C (54.8%), followed by Group B (16.7%) and Group E (14.3%). Antimicrobial susceptibility results revealed that most isolates were resistant to ampicillin (64.3%) and tetracycline (61.9%). Eighteen isolates (43.9%) exhibited multidrug resistant (MDR). The most frequent pattern of MDR was AMP-TE-SXT (19.1%). In summary, 100% of the minced pork samples from food retail outlets around the University of Phayao were contaminated with Salmonella, and 43.9% of the isolates were characterized to have a multidrug resistant phenotype.
Keywords: Salmonella, Minced pork, Serogrouping, Antimicrobial resistance
References
Angkititrakul, S., Chomvarin, C., Chaita, T., Kanistanon, K., & Waethewutajarn, S. (2005). Epidemiology of antimicrobial resistance in Salmonella isolated from pork, chicken meat and humans in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 36(6), 1510-1515.
Angulo, F. J., Nargund, V. N., & Chiller, T. C. (2004). Evidence of an association between use of anti-microbial agents in food animals and anti-microbial resistance among bacteria isolated from humans and the human health consequences of such resistance. Journal of veterinary medicine. B, Infectious diseases and veterinary public health, 51, 374-379.
Berends, B. R., Van, K. F., Snijders, J. M. A., & Mossel, D. A. A. (1997). Identification and quantification of risk factors regarding Salmonella spp. on pork carcasses. International Journal of Food Microbiology, 36, 199–206.
Bilhmad, U., Yoidam, S., Bhumibhamon, T., Thongnoon, P., & Anan, P. (2007). Serovars and antimicrobial drug resistance of Salmonella isolated from pork and chicken meat in Southern Thailand, Thai-NIAH eJournal, 2(1), 27-37. Retrieved from http://niah.dld.go.th/th/files/ejournal/v02n1t04.pdf.
Bureau of Quality and Safety of Food. (2017). The standards of microbiological quality of food and food containers. Bangkok, Thailand: P2 Design & Print.
FAO/WHO. (2016). Interventions for the control of non-typhoidal Salmonella spp. in beef and pork. Rome, Italy: Dennis Kunkel Microscopy.
Glynn, M. K., Bopp, C., Dewitt, W., Dabney, P., Mokhtar, M., & Angulo, F. J. (1998). Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States. The New England Journal of Medicine, 338, 1333–1338.
Hanson, R., Kaneene, J. B., Padungtod, P., Hirokawa, K., & Zeno, C. (2002). Prevalence of Salmonella and E. coli, and their resistance to antimicrobial agents, in farming communities in Northern Thailand, The Southeast Asian Journal of Tropical Medicine and Public Health, 33, 120-125.
International Organization for Standardization (ISO) 6579. (2002). Microbiology of food and animal feeding stuff-horizontal method for the detection of Salmonella. Geneva, Switzerland: ISO.
Legnani, P., Leoni, E., Berveglieri, M., Mirolo, G., & Alvaro, N. (2004). Hygienic control of mass catering establishments, microbiological monitoring of food and equipment. Food Control, 15, 205-211.
Lertworapreecha, M., Sutthimusik, S., & Tontikapong, K. (2013). Antimicrobial resistance in Salmonella enterica isolated from pork, chicken, and vegetables in Southern Thailand. Jundishapur Journal of Microbiology, 6(1), 36-41.
Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., & Monnet, D. L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3), 268-281.
Niyomdecha, N., Mungkornkaew, N., & Samosornsuk, W. (2016). Serotypes and antimicrobial resistance of Salmonella enterica isolated from pork, chicken meat and lettuce, Bangkok and Central Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 47(1), 31-39.
Pires, S. M., Vieira, A. R., Hald, T., & Cole, D. (2014). Source attribution of human salmonellosis: an overview of methods and estimates. Foodborne pathogens and disease, 11(9), 667–676.
Sakdinun, P., Naksuntorn, S., & Julagivansujarit, J. (n.d.). Detection of Salmonella contaminations in pork, chicken and beef in the western part of Thailand. Retrieved from http://niah.dld.go.th/th/files/ research/49.pdf.
Sanguankiat, A., Pinthong, R., Padungtod, P., Baumann, M. P. O., Zessin, K. H., Srikitjakarn, L., & Fries, R. (2010). A cross-sectional study of Salmonella in pork products in Chiang Mai, Thailand. Foodborne Pathogens and Disease, 7(8), 873-878.
Sirivan, C., Arsayuth, K., Thongsawatwong, E., & Paugtes, P. (1996). Outbreak of Salmonellosis from swine to man: Proceedings of the 34th Kasetsart University annual conference, 30 January-1 February 1996 (pp. 419-423). Bangkok: Thailand.
Sithigon, D., & Angkititrakul, S. (2011). Prevalence of Salmonella isolated from pigs, pig carcasses, water and workers at slaughterhouses in Khon Kaen province. KKU Veterinary Journal, 21(1), 33-40.
USDA. (1999). Generic HACCP model for poultry slaughter. Washington, D.C.: Food Safety and Inspection Service.
Van der Gaag, M. A., Saatkamp, H. W., Backus, G. B., Beek, P. V., & Huirne, R. B. (2004). Cost effectiveness of controlling Salmonella in the pork chain. Food Control, 15, 173-80.
Yamane, T. (1967). Statistics, An introductory analysis (2nd ed.). New York: Harper and Row.
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