Effects of Growth Regulators Produced by Methylobacterium radiotolerans Ed5-9 and Crude Antimicrobial Agents Extracted from Streptomyces TM32 on Tissue Culture of Gymnema inodorum (Lour.) Decne.
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Published
Sep 24, 2018
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Abstract
Since the demand for medicinal plant in pharmaceutical industries as plant based raw materials is becoming greater in quantity, the propagation of large number of plant in relatively short period of time can be produced by tissue technique. However, one of the most serious problems of plant cell and tissue culture is microbial contamination. The effectiveness of crude plant growth promoting substances extracted from the isolates Methylobacterium radiotolerans ED5-9 (MIAA) and the Streptomyces TM32 (SCE) for plant growth and development on tissue culture of the G. inodorum, a medicinal plant widely used in Northern Thailand, was therefore investigated. After incubation for 4 weeks, cooperation of the MIAA and SCE in basal liquid medium condition showed significantly (p < 0.05) higher increase in the length of shoot tip of explants than those semi-solid medium. High levels of IAA from both the M. radiotolerans ED5-9 and the Streptomyces TM32 caused reduction of shoot growth and root development when compared with the control treatment consisted of the MIAA alone. The measurement of greenness of leaves (SPAD readings) showed lower values from the liquid medium than that of the semi-solid treatments. The active antioxidant activity and total phenolic content accumulated in the plantlets of G. inodorum in semi-solid cultured plantlets were at a higher level than those in liquid treatment. Moreover, all these of SCE treatments, which contained crude antifungal agents, had no microbial contamination appearance during incubation. This study suggested that beneficial effects of the growth promoting substances producing, particularly IAA less than 0.01 mg/L, from both the M. radiotolerans ED5-9 and the Streptomyces TM32 or even from the M. radiotolerans ED5-9 alone might have potential for use as bio-fertilize or anti-microbial agent on micro-propagated Chiang Da, G. inodorum, in the semi-solid medium condition.
Keywords: Gymnema inodorum; Methylobacterium radiotolerans; plant growth promoting substances; Streptomyces; tissue culture
References
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Bashan, Y., & Holguin, G. (1998). Proposal for the division of plant growth-promoting rhizobacteria into two classifications: Biocontrol-PGPB (plant growth-promoting bacteria) and PGPB. Soil Biology and Biochemistry, 30, 1225–1228.
Bauthet, S., Nakaew, N., Chidburee, A., & Sarin, S. (2014). Effect of plant growth promoting substances produced by pink pigmented facultative methylotrophs on tissue culture of Rauvolfia serpentina (L.) Benth. ex Kurz. In: Division of research, Naresuan University (Eds.), Proceeding of 10th National Naresuan University Research Conference (pp. 150-157). Phitsanulok: Thailand.
Bouizgarne, B., El Hadrami, I., & Ouhdouch, Y. (2006) Novel production of isochainin by a strain of Streptomyces sp. isolated from rhizosphere soil of the indigenous Moroccan plant Argania spinosa L. World Journal of Microbiology and Biotechnology, 22, 423-429.
Cassells, A. C. (1991). Problems in tissue culture: Culture contamination. In: P.C. Debergh, & R.H. Zimmerman (Eds.), Micropropagation (pp. 31–44) Netherlands: Kluwer Academic Publishers.
Cassells, A. C. (2001). Contamination and its impact in tissue culture. Acta Hort, 560, 353-359.
Chapman, S. C., & Barreto, H. J. (1997). Using a chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth. Agronomy Journal, 89, 557–562.
Chidburee, A., Putawanchai, P., & Sarin, S. (2015). Effect of IAA concentration from fermentation broth with culture condition on growth and development of Chiang-da in vitro. King Mongkut’s Agricultural Journal, 1, 42-46.
Compant, S., Clément, C., & Sessitsch, A. (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry, 42, 669-678.
Floegel, A., Kim, D., Chung, S., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24, 1043–1048.
George, E. F. (2008). Plant Growth Regulators I: Introduction; Auxins, their Analogues and Inhibitors. In: E.F. George, M.A. Hall, & J. Deklerk (Eds.), Plant Propagation by Tissue Culture (3rd ed., pp. 206–217). The Netherlands: Springer.
Glickmann. E, & Dessaux, Y. (1995). A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61, 793-796.
Hendry, G. A .F., & Price, A. H. (1993). Stress indicators: chlorophylls and carotenoids In: G. A. F., Hendry, & J. P. Grime (Eds.), Methods in Comparative Plant Ecology (pp. 148–152) London: Chapman & Hall.
Ivanova, E.G., Doronina, N.V., & Trotsenko, Yu.A. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology, 70, 392-397.
Kim, D. O., Chun, O. K., Kim, Y. J., Moon, H. Y., & Lee, C. Y. (2003) Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry, 51, 6509–6515.
Kloepper, J. W., & Schroth, M. N. (1978). Plant growth-promoting rhizobacteria on radishes. In: Station de Pathologie Vegetale et Phyto-Bacteriologie (Ed.), Proceedings of the 4th International Conference on Plant Pathogenic Bacteria (pp. 879-882) vol. II. Tours, France: Gilbert-Clarey.
Klungsupya, P., Muangman, T., Theangtrong, N., Khayungarnnawee, A., Phatvej, W., Thisayakorn, K., Rerk-Am, U., Sematong, T., Trangvacharakul, S., & Arunpairojana, V. (2008). Antioxidant and antihyperglycemic activities of Gymnema inodorum Dence. In: Proceeding of the 8th NRCT-JSPS Joint Seminar Innovative Research in Natural Products for Sustainable Development (pp. 207-209). Bangkok: Thailand.
Leifert, C., & Cassells, A.C. (2001). Microbial hazards in plant tissue and cell culture. In Vitro Cellular Development and Biology – Plant, 38, 133-138.
Mathias, P. J., Alderson, P. G., & Leakey, R. R. B. (1987). Bacterial contamination in tropical hardwood cultures. Acta Horticulturae, 212, 43-48.
Mbah, E. I., & Wakil, S. M. (2012). Elimination of bacteria from In vitro yam tissue cultures using antibiotics. Journal of Plant Pathology, 94, 53-58.
Mensor, L. L., Menezes, F. S, Leitao, G.G., Reis, A.S., dos Santos, T.C., Coube, C.S., & Leitao, S.G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research, 15, 127-130.
Min, Y. J., Jung, H. Y, Kang, S. M., Kim, Y. D., Kang, Y. M., Park, D. J., … Choi, M. S. (2007). Production of tropane alkaloids by small scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresource Technology, 98, 1748-1753.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
Nachtigall, J., Schulz, D., Beil, W., Sussmuth, R. D., & Fiedler, H. P. (2010). Aranciamycin anhydride, a new anthracycline-type antibiotic isolated from Streptomyces sp. Tu6384. The Journal of Antibiotics, 63, 397-399.
Nakaew, N., Rangjaroen, C., & Sungthong, R (2015). Utilization of rhizospheric Streptomyces for biological control of Rigidoporus sp. causing white root disease in rubber tree. European Journal of Plant Pathology, 142, 93-105. http://dx.doi.org/ 10.1007/s10658-015-0592-0
Navarro, L., Dunoyer, P., Jay, F., Arnold, B,, Dharmasiri, N., Estelle, M., … Jones, J. D. G. (2006). A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science,
312, 436–439.
Parimala Devi, R., Sundaram, S. P., & Poorniammal, R. (2010). Effect of facultataive methylotrphs on tissue culturing of rice. Asian Journal of Biological Science, 4, 207-209.
Prombunchachai, T. (2015). Potential of Pink-Pigmented Facultative Methylotrophs (PPFMs) to enhance Growth of Murdannia loriformis (Hassk.) R. Rao & Kammathy in tissue culture. Naresuan University, Thailand.
Sabale, S. N., Patil, D. M., Gokhale, N. B., Sawardekar, S. V., & Sawant, S. S. (2015). Elimination of bacterial contamination by using antibiotics in micropropagation of banana (Musa spp.) cv. Grand Naine. Cell and Tissue Research, 15, 5111-5115.
Saleh-Lakha, S., & Glick, B. R. (2006). Plant growth-promoting bacteria. In: J.D. van Elsas, J.K. Jansson, & J.T. Trevors (Eds), Modern soil microbiology (pp. 503-520). Boca Raton, FL/UK: CRC/Thomson Publishing.
Sarin, S., Prombunchachai, T., Nakaew, N., & Chidburee, A. (2013). Isolation of indole acetic acid producing Pink Pigmented Facultative Methylotrophs (PPFMs) from Murdannia loriformis (Hassk.) R. Rao & Kammathy. Naresuan University Journal, 21, 14-24.
Shimizu, K., Ozeki, M., Tanaka, K., Itoh, K., Nakajyo, S., Urkawa, N., & Atsuchi, M. (1997). Suppression of glucose absorption by extracts from the leaves of Gymnema inodorum. The Journal of Veterinary Medical Science, 59, 753-757.
Shimizu, K., Ozeki, M., lino, A., Nakajyo, S., Urkawa, N., & Atsuchi, M. (2001). Structure activity relationship of triterpenoid derivatives extracted from Gymnema inodorum leaves on glucose absorption. The Japanese Journal of Pharmacology, 86, 223-229.
Shirokikh, I. G., Shupletsova, O. N., & Shirokikh, A. A. (2007). Assessment of the effect of Methylotrophic bacteria on plants In vitro. Russian Agricultural Sciences, 33, 308-310.
Sritontip, P., Sritontip, C., & Pinchai, J. (2016). Effects of Pig Manure on some Chemical Properties of Soil, Consumable Parts and Antioxidant Activitiesin Gymnema inodorum (Lour.) Decne. Songklanakarin Journal of Plant Science, 3, 43-49.
Suthar, R. K., Habibi, N., & Purohi, S. D. (2011). Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb. Indian Journal of Biotechnology, 10, 224-227.
Umamaheswari, C., Ambethkar, A., Margaret, F. S, & Selvaraj, N. (2014). In Vitro Root Culture From Roots And Leaf Explants Of Luffa Acutangula(L.) Roxb. International Journal of Innovative Research in Science, Engineering and Technology, 3, 16886-16895. http://dx.doi.org/10.15680/ IJIRSET.2014.0310068
Van den Houwe, I., & Swennen, R. (2000). Characterization and control of bacterial contaminants in vitro cultures of banana (Musa spp.). Acta Horticulterae, 532, 69-79.
Zoecklein, B. W., Fugelsang, K. C., Gum, B. H., & Nury, F. S. (1995). Wine Analysis and Production. New York: Chapman & Hall.
Bashan, Y., & Holguin, G. (1998). Proposal for the division of plant growth-promoting rhizobacteria into two classifications: Biocontrol-PGPB (plant growth-promoting bacteria) and PGPB. Soil Biology and Biochemistry, 30, 1225–1228.
Bauthet, S., Nakaew, N., Chidburee, A., & Sarin, S. (2014). Effect of plant growth promoting substances produced by pink pigmented facultative methylotrophs on tissue culture of Rauvolfia serpentina (L.) Benth. ex Kurz. In: Division of research, Naresuan University (Eds.), Proceeding of 10th National Naresuan University Research Conference (pp. 150-157). Phitsanulok: Thailand.
Bouizgarne, B., El Hadrami, I., & Ouhdouch, Y. (2006) Novel production of isochainin by a strain of Streptomyces sp. isolated from rhizosphere soil of the indigenous Moroccan plant Argania spinosa L. World Journal of Microbiology and Biotechnology, 22, 423-429.
Cassells, A. C. (1991). Problems in tissue culture: Culture contamination. In: P.C. Debergh, & R.H. Zimmerman (Eds.), Micropropagation (pp. 31–44) Netherlands: Kluwer Academic Publishers.
Cassells, A. C. (2001). Contamination and its impact in tissue culture. Acta Hort, 560, 353-359.
Chapman, S. C., & Barreto, H. J. (1997). Using a chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth. Agronomy Journal, 89, 557–562.
Chidburee, A., Putawanchai, P., & Sarin, S. (2015). Effect of IAA concentration from fermentation broth with culture condition on growth and development of Chiang-da in vitro. King Mongkut’s Agricultural Journal, 1, 42-46.
Compant, S., Clément, C., & Sessitsch, A. (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry, 42, 669-678.
Floegel, A., Kim, D., Chung, S., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24, 1043–1048.
George, E. F. (2008). Plant Growth Regulators I: Introduction; Auxins, their Analogues and Inhibitors. In: E.F. George, M.A. Hall, & J. Deklerk (Eds.), Plant Propagation by Tissue Culture (3rd ed., pp. 206–217). The Netherlands: Springer.
Glickmann. E, & Dessaux, Y. (1995). A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61, 793-796.
Hendry, G. A .F., & Price, A. H. (1993). Stress indicators: chlorophylls and carotenoids In: G. A. F., Hendry, & J. P. Grime (Eds.), Methods in Comparative Plant Ecology (pp. 148–152) London: Chapman & Hall.
Ivanova, E.G., Doronina, N.V., & Trotsenko, Yu.A. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology, 70, 392-397.
Kim, D. O., Chun, O. K., Kim, Y. J., Moon, H. Y., & Lee, C. Y. (2003) Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry, 51, 6509–6515.
Kloepper, J. W., & Schroth, M. N. (1978). Plant growth-promoting rhizobacteria on radishes. In: Station de Pathologie Vegetale et Phyto-Bacteriologie (Ed.), Proceedings of the 4th International Conference on Plant Pathogenic Bacteria (pp. 879-882) vol. II. Tours, France: Gilbert-Clarey.
Klungsupya, P., Muangman, T., Theangtrong, N., Khayungarnnawee, A., Phatvej, W., Thisayakorn, K., Rerk-Am, U., Sematong, T., Trangvacharakul, S., & Arunpairojana, V. (2008). Antioxidant and antihyperglycemic activities of Gymnema inodorum Dence. In: Proceeding of the 8th NRCT-JSPS Joint Seminar Innovative Research in Natural Products for Sustainable Development (pp. 207-209). Bangkok: Thailand.
Leifert, C., & Cassells, A.C. (2001). Microbial hazards in plant tissue and cell culture. In Vitro Cellular Development and Biology – Plant, 38, 133-138.
Mathias, P. J., Alderson, P. G., & Leakey, R. R. B. (1987). Bacterial contamination in tropical hardwood cultures. Acta Horticulturae, 212, 43-48.
Mbah, E. I., & Wakil, S. M. (2012). Elimination of bacteria from In vitro yam tissue cultures using antibiotics. Journal of Plant Pathology, 94, 53-58.
Mensor, L. L., Menezes, F. S, Leitao, G.G., Reis, A.S., dos Santos, T.C., Coube, C.S., & Leitao, S.G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research, 15, 127-130.
Min, Y. J., Jung, H. Y, Kang, S. M., Kim, Y. D., Kang, Y. M., Park, D. J., … Choi, M. S. (2007). Production of tropane alkaloids by small scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresource Technology, 98, 1748-1753.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
Nachtigall, J., Schulz, D., Beil, W., Sussmuth, R. D., & Fiedler, H. P. (2010). Aranciamycin anhydride, a new anthracycline-type antibiotic isolated from Streptomyces sp. Tu6384. The Journal of Antibiotics, 63, 397-399.
Nakaew, N., Rangjaroen, C., & Sungthong, R (2015). Utilization of rhizospheric Streptomyces for biological control of Rigidoporus sp. causing white root disease in rubber tree. European Journal of Plant Pathology, 142, 93-105. http://dx.doi.org/ 10.1007/s10658-015-0592-0
Navarro, L., Dunoyer, P., Jay, F., Arnold, B,, Dharmasiri, N., Estelle, M., … Jones, J. D. G. (2006). A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science,
312, 436–439.
Parimala Devi, R., Sundaram, S. P., & Poorniammal, R. (2010). Effect of facultataive methylotrphs on tissue culturing of rice. Asian Journal of Biological Science, 4, 207-209.
Prombunchachai, T. (2015). Potential of Pink-Pigmented Facultative Methylotrophs (PPFMs) to enhance Growth of Murdannia loriformis (Hassk.) R. Rao & Kammathy in tissue culture. Naresuan University, Thailand.
Sabale, S. N., Patil, D. M., Gokhale, N. B., Sawardekar, S. V., & Sawant, S. S. (2015). Elimination of bacterial contamination by using antibiotics in micropropagation of banana (Musa spp.) cv. Grand Naine. Cell and Tissue Research, 15, 5111-5115.
Saleh-Lakha, S., & Glick, B. R. (2006). Plant growth-promoting bacteria. In: J.D. van Elsas, J.K. Jansson, & J.T. Trevors (Eds), Modern soil microbiology (pp. 503-520). Boca Raton, FL/UK: CRC/Thomson Publishing.
Sarin, S., Prombunchachai, T., Nakaew, N., & Chidburee, A. (2013). Isolation of indole acetic acid producing Pink Pigmented Facultative Methylotrophs (PPFMs) from Murdannia loriformis (Hassk.) R. Rao & Kammathy. Naresuan University Journal, 21, 14-24.
Shimizu, K., Ozeki, M., Tanaka, K., Itoh, K., Nakajyo, S., Urkawa, N., & Atsuchi, M. (1997). Suppression of glucose absorption by extracts from the leaves of Gymnema inodorum. The Journal of Veterinary Medical Science, 59, 753-757.
Shimizu, K., Ozeki, M., lino, A., Nakajyo, S., Urkawa, N., & Atsuchi, M. (2001). Structure activity relationship of triterpenoid derivatives extracted from Gymnema inodorum leaves on glucose absorption. The Japanese Journal of Pharmacology, 86, 223-229.
Shirokikh, I. G., Shupletsova, O. N., & Shirokikh, A. A. (2007). Assessment of the effect of Methylotrophic bacteria on plants In vitro. Russian Agricultural Sciences, 33, 308-310.
Sritontip, P., Sritontip, C., & Pinchai, J. (2016). Effects of Pig Manure on some Chemical Properties of Soil, Consumable Parts and Antioxidant Activitiesin Gymnema inodorum (Lour.) Decne. Songklanakarin Journal of Plant Science, 3, 43-49.
Suthar, R. K., Habibi, N., & Purohi, S. D. (2011). Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb. Indian Journal of Biotechnology, 10, 224-227.
Umamaheswari, C., Ambethkar, A., Margaret, F. S, & Selvaraj, N. (2014). In Vitro Root Culture From Roots And Leaf Explants Of Luffa Acutangula(L.) Roxb. International Journal of Innovative Research in Science, Engineering and Technology, 3, 16886-16895. http://dx.doi.org/10.15680/ IJIRSET.2014.0310068
Van den Houwe, I., & Swennen, R. (2000). Characterization and control of bacterial contaminants in vitro cultures of banana (Musa spp.). Acta Horticulterae, 532, 69-79.
Zoecklein, B. W., Fugelsang, K. C., Gum, B. H., & Nury, F. S. (1995). Wine Analysis and Production. New York: Chapman & Hall.
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SARIN, Siripun; NAKAEW, Nareeluk; CHIDBUREE, Aphichat.
Effects of Growth Regulators Produced by Methylobacterium radiotolerans Ed5-9 and Crude Antimicrobial Agents Extracted from Streptomyces TM32 on Tissue Culture of Gymnema inodorum (Lour.) Decne..
Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 26, n. 3, p. 52-62, sep. 2018.
ISSN 2539-553X.
Available at: <https://www.journal.nu.ac.th/NUJST/article/view/Vol-26-No-3-2018-52-62>. Date accessed: 25 apr. 2024.
doi: https://doi.org/10.14456/nujst.2018.4.