Mycelial Cultivation of 4 Edible Mushrooms from Khao Kra-Dong Volcano Forest Park, Thailand


Tepupsorn Saensuk Suteera Suntararak


     The edible mushrooms become one of the world’s most expensive foods and have a global market measured in Thailand. In Thailand, the fruiting body of all occurs once a year during rainy season in June – August. So, the objective of this research was to study the optimal mycelial conditions of 4 edible mushrooms collected from Khao Kra-Dong Volcano Forest Park in Thailand: Russula cyanoxantha, Heimiell retispora, Russula virescens and Boletus colossus. The highest mycelial growth of R. virescens and B. colossus were with potato dextrose agar (PDA), followed by potato dextrose agar with 2% volcanic soil (PDA+2% S). The best structures of R. cyanoxantha and H. retispora used for culturing on medium were cap, stalk and spore, respectively. For R. Virescens and B. colossus, the best structures used for culturing on medium were stalk, cap and spore, respectively. The highest colony diameter of R. Cyanoxantha on PDA+2% S with cap was 68.00+1.00 mm. For H. retispora, the highest colony diameters on PDA+2%S with cap and PDA with stalk were 87.00+1.00 mm. and 67.33+1.53 mm., respectively. For R. virescens and B. colossus, the highest colony diameters on PDA with stalk were 91.33+2.08 mm. and 87.00+1.00 mm., respectively. The optimal temperature and pH value for mycelial growth were at 30 °C with pH 7 after 7 days of incubation.

Keywords: mushroom cultivation, edible mushrooms, volcanic soil


Abdulhadi, M. A., & Hassan, I. A. A. (2013). Effect of Sterilization Method and Supplementation on the Yield and Storage Life of Oyster Mushroom Cultivated on Date Palm by Products. Dialy J. Agric. Sci, 5, 170–181.

Adebayo-Tayo, B. C., Jonathan, S. G., Popoola, O. O., & Egbomuche, R. C. (2011). Optimization of Growth Conditions for Mycelial Yield and Xxopoly Saccharride Production by Pleurotus Ostreatus Cultivated in Nigeria. Afr. J. Microbiol. Res., 5, 2130-2138

Barros, L., Ferreira, M-J., & Queiros, B. (2007) Total Phenols, Ascorbic Acid, β-Carotene and Lycopene in Portuguese Wild Edible Mushrooms and Their Antioxidant Activities. Food Chemistry, 103, 413 – 419.

Brosius, F. (2008). SPSS 16. (1ST ed.). Heidelberg: Redline GmbH.

Chang, S. T., (2000). Global Impact of Edible and Medicinal Mushrooms on Human Welfare in the 21st Century: No Green Revolution. Int. J. Med. Mushrooms, 1, 1–7.

FAO (Food and Agriculture Organization of the United Nations). (1990). Technical Guidelines for Mushroom Growing in the Tropics. Food and Agriculture Organization of the United Nations, Rome: The European Union

Gbolagade, J. S., Fasidi, I. O., Ajayi, E. J., & Sobowale, A. A. (2006). Effect of Physicochemical Factors and Semi-Synthetic Media on Vegetative Growth of Lentinus Subnudus (Berk.), an Edible Mushroom from Nigeria. Food Chem, 99, 742-747.

Heleno, S. A., Barros, L., Sousa, M. J., Martins, A., & Ferreira, I. C. F. R. (2009). Study and Characterization of Selected Nutrients in Wild Mushrooms from Portugal by Gas Chromatography and High Performance Liquid Chromatography. Microchemical Journal, 93, 195 –199.

Janjira, W., Paiboolya, K., & Saisamorn, L. (2014). Effects of Different Culture Media, Carbon and Nitrogen Sources and Solid Substrates on Growth of Termitomyces Mushrooms. Chiang Mai J. Sci, 41(3), 542-556.

Kalac, P. (2009). Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food chemistry, 113, 9–16.

Kumla, J., Danell, E., & Lumyong, S. (2014). Improvement of Yield for a Tropical Black Bolete, Phlebopus Portentosus, Cultivation in Northern Thailand. Mycoscience, 56(1), 1–4.

Lai, W. H., Siti Murni, M. J., Fauzi, D., Abas Mazni, O., & Saleh, N. M. (2011). Optimal Culture Conditions for Mycelial Growth of Lignosus Rhinocerus. Mycobiology, 39, 92–95.

Leela, M. R., Kevin, D. H., Ekachai, C., & Sunita C. (2014). Optimal Mycelial Conditions and Spawn Production for the Domestication of Macrolepiota Deters. The 26th Annual Meething of Thai Society for Biology and International conference: Thailand.

Leela, M. R., Sunita, C., & Kevin, D. H. (2016). First Successful Cultivation of the Edible Mushroom Macrolepiota Dolichaula in Thailand. Chiang Mai J. Sci. 43(5), 959-971.

Nasim, G., Malik, S. H., Bajwa, R., & Afzal, M. (2001). Effect of Three Different Culture Media on Mycelia Growth of Oyster and Chinese Mushroom. J. Biol. Sci, 1, 1130-1133.

Ouzouni, P. K, Petridis, D., Koller, W. D., & Riganakos, K. A. (2009). Nutritional Value and Metal Content of Wild Edible Mushrooms Collected from West Macedonia and Epirus, Greece. Food Chemistry, 115, 1575–1580.

Rajarathnam, S., & Bano, Z., (1991). Bio-logical Utilization of Edible Fruiting Fungi III. In D. Aurora, K. Mukerji, M. E. Dekker (Eds.), Handbook of Applied Mycology Inc., (pp.241–292). New York, NY,: CAB International.

Shim, S. M., Oh, Y. H., Lee, K. R., & Lee, T. S. (2005). The Characteristics of Cultural Conditions for the Mycelial Growth of Macrolepiota Procera. Mycobiology, 33, 15–18.

Wei, T. Z., Yao, Y. J., Wang, B., & Pegler, D. N. (2004). Termitomyces Bulborhizus Sp. Nov. from China, with a Key to Allied Species. Mycol. Res, 108, 1458-1462.

Mushroom cultivation, Edible Mushrooms, Volcanic soil Naresuan University; Science technology
Research Articles


How to Cite
SAENSUK, Tepupsorn; SUNTARARAK, Suteera. Mycelial Cultivation of 4 Edible Mushrooms from Khao Kra-Dong Volcano Forest Park, Thailand. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 26, n. 2, p. 38-45, june 2018. ISSN 2539-553X. Available at: <>. Date accessed: 17 july 2018.