Hydroxyapatite Prepared by Co-Precipitation with Calcium Carbonate: Effects of Digested Calcium Carbonate and Phosphate Sources


Wallika Suksomrana and Robert Molloy


     The Hydroxyapatite (HAP) was prepared by co-precipitation of calcium carbonate (CaCO3) with various orthophosphate sources such as phosphoric acid (H3PO4), ammonium dihydrogen phosphate (NH4H2PO4) and diammonium hydrogen phosphate ((NH4)2HPO4) under ambient conditions. CaCO3was digested by 20% HNO3 to produce calcium cation which reacted with the orthophosphate ion to form hydroxyapatite. All XRD patterns closely matched with the HAP reference data. There was no change in the sharpness of the diffraction peaks and little or no change in the peak intensity was observed when compared to various phosphate sources (H3PO4, NH4H2PO4and (NH4)2HPO4). The FTIR spectrum showed without the characteristic bands of B-type CO32- substitution at 1421–1473 cm-1 (v3) and 874 cm-1(v2). Moreover, the FTIR spectrum showed without the characteristic peak of A-type CO32-substitution at 1540 cm-1. The precipitation of HAP from digested CaCO3 solution occurred at 3 hours, while the reaction from non-digested CaCO3 solution occurred at 18 hours. The digestion of CaCO3 reduced the preparation time of HAP and also decreased the CO32-substitution in HAP.


An, G. H., Wang, H. J., Kimb, B. H., Jeong, Y. G., & Choa, Y. H. (2007). Fabrication and characterization of a hydroxyapatite nanopowder by ultrasonic spray pyrolysis with salt-assisted decomposition. Materials Science and Engineering A, 449–451, 821–824. Retrieved from http://dx.doi:10.1016/j.msea.2006.02.436

Bezzi, G., Celotti, G., Landi, E., La Torretta, T. M. G., Sopyan, I., & Tampieri, A. (2003). A novel sol-gel technique for hydroxyapatite preparation. Materials Chemistry and Physics, 78, 816–824.

Bouyer, E., Gitzhofer, F., & Boulos, M. I. (2000). Morphological study of hydroxyapatite nanocrystal suspension. Journal of Material Science: Materials in Medicine, 11, 523-531.

Hui, P., Meena, S. L., Singh, G., Agarawal, R. D., & Prakash, S. (2010). Synthesis of Hydroxyapatite Bio-Ceramic Powder by Hydrothermal Method. Journal of Minerals & Materials Characterization & Engineering, 9, 683-692.

Inthong, S., Tunkasiri, T., Eitssayeam, S., Pengpat, K., & Rujijanagul, G. (2013). Physical properties and bioactivity of nanocrystalline hydroxyapatite synthesized by a co-precipitation route. Ceramics International, 39, S533–S536. Retrieved from http://dx.doi.org/10.1016/j. ceramint.2012.10.129

Kim, Y., & Ohtsuki, C. (2016). Hydroxyapatite formation from calcium carbonate single crystal under hydrothermal condition: Effects of processing temperature. Ceramics International, 42, 1886–1890. Retrieved from http://dx.doi. org/10.1016/ j.ceramint.2015.09.156

Kong, L. B., Ma, J., & Boey, F. (2002). Nanosized hydroxyapatite powders derived from coprecipitation process. Journal of Materials Science, 37, 1131-1134.

Mahabole, M. P., Aiyer, R. C., Ramakrishna, C. V., Sreedhar, B., & Khairnar, R. S. (2005). Synthesis, characterization and gas sensing property of hydroxyapatite ceramic. Bulletin of Materials Science, 28(6), 535–545.

Minh, D. P., Lyczko, N., Sebei, H., Nzihou, A., & Sharrock, P. (2012). Synthesis of calcium hydroxyapatite from calcium carbonate and different orthophosphate sources: A comparative study. Materials Science and Engineering B, 177, 1080–1089. Retrieved from http://dx.doi.org/ 10.1016/j.mseb.2012.05.007

Nasiri-Tabrizi, B., Honarmandi, P., Ebrahimi-Kahrizsangi, R., & Honarmandi, P. (2009). Synthesis of nanosize single-crystal hydroxyapatite via mechanochemical method. Materials Letters, 63, 543–546. Retrieved from http://dx.doi: 10.1016/j.matlet.2008.11.030

Piantone, P., Bodénan, F., Derie, R., & Depelsenaire, G. (2003). Monitoring the stabilization of municipal solid waste incineration fly ash by phosphation: mineralogical and balance approach. Waste Management, 23, 225-243.

Recillas, S., Rodríguez-Lugo, V., Montero, M. L., Viquez-Cano, S., Hernandeze, L., & Castaño, V. M. (2012). Studies on the precipitation behavior of calcium phosphate solutions. Journal of Ceramic Processing Research, 13(1), 5-10.

Sonoda, K., Furuzono, T., Walsh, D., Sato, K., & Tanaka, J. (2002). Influence of emulsion on crystal growth of hydroxyapatite. Solid State Ionics, 151, 321 – 327.

Verwilghen, C., Chkir, M., Rio, S., Nzihou, A., Sharrock, P., & Depelsenaire, G. (2009). Convenient of Calcium Carbonate to Hydroxyapatite at Ambient Pressure. Material Science and Engineering C, 29, 771-773. Retrieved from http://dx.doi:10.1016/j.msec. 2008.07.007

Waheeda, S., Sultana, M., Jamila, T., & Hussainb, T. (2013). Comparative analysis of hydroxyapatite synthesized by sol-gel, ultrasonication and microwave assisted technique. Proceedings of the International Conference on Solid State Physics 2013 (ICSSP’13), 2(2015), 5477-5484. Retrieved from http://dx.doi: 10.1016/j.matpr.2015.11.073

Hydroxyapatite, Calcium carbonate, Co-precipitation
Research Articles


How to Cite
ROBERT MOLLOY, Wallika Suksomrana and. Hydroxyapatite Prepared by Co-Precipitation with Calcium Carbonate: Effects of Digested Calcium Carbonate and Phosphate Sources. Naresuan University Journal: Science and Technology (NUJST), [S.l.], v. 25, n. 1, p. 57-66, feb. 2017. ISSN 2539-553X. Available at: <http://www.journal.nu.ac.th/NUJST/article/view/1676>. Date accessed: 19 apr. 2019.